DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele

Systems Biology in Reproductive Medicine
M BahreinianMohammad H Nasr-Esfahani

Abstract

This study aimed to assess and compare global DNA methylation (GDM) between fertile men and infertile men with varicocele. In addition, we evaluated the correlations between DNA methylation with reactive oxygen species (ROS), protamine deficiency, and DNA integrity. Semen samples were collected from 44 men with grades II and III varicocele, and 15 fertile men for assessment of semen parameters, DNA methylation, DNA fragmentation, oxidative stress, and protamine deficiency. Samples were evaluated by the World Health Organization (WHO) criteria, immunostaining, the TUNEL assay, 2', 7'-dichlorodihydrofluorescein diacetate (DCFH-DA) staining, and chromomycin A3 (CMA3) staining. Semen parameters were significantly lower in individuals with varicocele compared to fertile men. The percentage of GDM and intensity of DCFH were reduced and the percentages of DCFH, TUNEL, and CMA3 positive sperm significantly increased in individuals with varicocele compared to fertile men. Correlation analysis revealed a negative significant relation between DNA methylation and DNA fragmentation, but not with the degree of protamine deficiency and ROS production. The results have shown that individuals with varicocele show increased DNA susceptibility to...Continue Reading

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Citations

Sep 11, 2016·Journal of Assisted Reproduction and Genetics·Elham Janghorban-LarichehMohammad H Nasr-Esfahani
May 13, 2018·BJU International·Parisa MohammadiMohammad H Nasr-Esfahani
Jul 25, 2018·Andrologia·Erfaneh ShayganniaMohammad H Nasr-Esfahani
Nov 27, 2018·Andrologia·Rana Ghandehari-AlavijehMohammad H Nasr-Esfahani
Jan 3, 2019·Systems Biology in Reproductive Medicine·Viviane Paiva SantanaRosana Maria Dos Reis
Mar 29, 2019·Andrologia·Sedigheh NematollahiMohammad Salehi
Apr 27, 2019·Human Fertility : Journal of the British Fertility Society·Tahereh RahiminiaAli Reza Talebi
Oct 4, 2019·Systems Biology in Reproductive Medicine·Miriama ŠtiavnickáJan Nevoral
Oct 21, 2018·Reproductive Biology and Endocrinology : RB&E·Pamela Tello-MoraBetzabet Quintanilla-Vega
Aug 14, 2020·Human Reproduction Update·Fredrika ÅseniusSarah J Marzi
Jul 26, 2018·Frontiers in Endocrinology·Nicola ColacurciFrancesco Lombardo
Mar 15, 2019·Scientific Reports·Sahar TahamtanMohammad Hosseini Nasr-Esfahani
Sep 20, 2020·Journal of Assisted Reproduction and Genetics·Tara Staley
May 20, 2017·Journal of Assisted Reproduction and Genetics·Viviane Paiva SantanaRosana Maria Dos Reis
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Oct 16, 2019·Andrologia·Hassan Hassani-BafraniMohammad Hossein Nasr-Esfahani
Aug 21, 2020·Basic and Clinical Andrology·Z MohammadiM H Nasr-Esfahani
Aug 11, 2020·The World Journal of Men's Health·Ashok AgarwalRupin Shah
Nov 5, 2020·Andrology·Mazdak RaziMohammad-Hossein Nasr-Eshafani
Jul 27, 2021·Andrologia·Zohreh Talebi-YazdabadiMohammad Hossein Nasr-Esfahani

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