PMID: 11309783Apr 20, 2001Paper

DNA image cytometry in the differential diagnosis of endocervical adenocarcinoma

Cancer
S BiesterfeldA Böcking

Abstract

The value of DNA image cytometry in the differential diagnosis of endocervical adenocarcinoma was tested on a series of 65 cases of normal endocervical cells (n = 25), inflammatory changes (n = 18), and endocervical adenocarcinoma (n = 22). The investigation was performed on gynecologic routine smears by using a television image analysis system MIAMED-DNA (Leica, Wetzlar, Germany), combined with an automated Leica Medilux microscope. First, the Papanicolaou stained specimens were rescreened, and the x/y coordinates of at least 150 endocervical nuclei were stored per case by using a scanning program. After restaining according to Feulgen, the epithelial cells were relocalized and the DNA content, and the nuclear area were determined. The DNA content of 25-30 squamous epithelial cells of intermediate type served as an internal standard for the normal diploid value in each case. Various DNA cytometric parameters and the mean nuclear area were calculated. For statistical analysis, the cases of adenocarcinoma (n = 22) were defined as positive, and the cases with normal endocervical epithelium or inflammatory changes (n = 43) were defined as negative. The presence of nuclei with a DNA content greater than 9c was observed exclusively ...Continue Reading

References

Sep 1, 1992·Gynecologic Oncology·U HørdingJ E Bock
Jan 1, 1991·Journal of Clinical Laboratory Analysis·D C Aziz, J B Peter
May 1, 1991·American Journal of Clinical Pathology·A LeminenM Lehtinen
Feb 1, 1991·Archives of Otolaryngology--head & Neck Surgery·J D Crissman, R J Zarbo
Feb 1, 1990·Geburtshilfe und Frauenheilkunde·R Bollmann
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Oct 1, 1995·The British Journal of Dermatology·S BiesterfeldA Böcking
Jan 1, 1994·Journal of Clinical Pathology·S BiesterfeldA Böcking
Jul 3, 1999·Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology·F GiroudA Böcking

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Citations

Dec 26, 2002·American Journal of Obstetrics and Gynecology·Loyd A WestMichele Follen
Dec 18, 2020·Diagnostic Cytopathology·Ane Francyne CostaFabiana Botelho de Miranda Onofre

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