DNA immobilisation procedures for surface plasmon resonance imaging (SPRI) based microarray systems

Biosensors & Bioelectronics
Ilaria MannelliMichael Canva

Abstract

Two different surface chemistries have been studied for the development of surface plasmon resonance imaging (SPRI) based DNA microarray affinity sensors: (1) 11-mercaptoundecanoic acid-poly(ethylenimine) (MUA-PEI) and (2) dextran procedures. The MUA-PEI method consists of assembling a multilayer on the basis of electrostatic interactions formed with: 11-mercaptoundecanoic acid (MUA), poly(ethylenimine) (PEI) and extravidin layers. The dextran procedure involves assembling a multilayer formed with 11-mercaptoundecanol, dextran and streptavidin layers, which are linked by covalent bonds. The oligonucleotide probes are immobilised onto the sensor surface as spots forming a matrix 14x14, which is spotted by a robot, while the target sequences are free in solution. The system allows the interaction (hybridisation) monitoring, in real-time and in parallel, of unlabeled oligonucleotide solution targets to oligonucleotide probes immobilised on a 196 spots matrix. Using oligonucleotides as probes and targets, both functionalised surfaces have been evaluated in view of their application to the diagnosis of gene mutations involved in human diseases. In particular, we demonstrate the ability to detect, in parallel, several mutations causi...Continue Reading

References

Nov 11, 1999·Journal of Molecular Recognition : JMR·B GoldsteinC Wofsy
Jan 29, 2002·Nucleic Acids Research·A W PetersonR M Georgiadis
Nov 17, 2004·The British Journal of Oral & Maxillofacial Surgery·J R Bowden, P A Brennan
Mar 9, 2005·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·S Van BiervlietA Christophe
Mar 31, 2005·Biosensors & Bioelectronics·B FouquéF Chatelain

❮ Previous
Next ❯

Citations

Jan 30, 2008·Analytical and Bioanalytical Chemistry·Sotirios S TragouliasTheodore K Christopoulos
Feb 16, 2010·Analytical and Bioanalytical Chemistry·Hany NasefCiara K O'Sullivan
Apr 9, 2013·Analytica Chimica Acta·Hana Šípová, Jiří Homola
Apr 2, 2013·Analytica Chimica Acta·Ulla KarhunenTero Soukka
Oct 28, 2008·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jul 31, 2009·IET Nanobiotechnology·S PaulA K Ray
May 24, 2012·The Analyst·Jérôme HottinMichael Canva
Mar 11, 2011·Nanotechnology·Dimitrios S ElenisTheodore K Christopoulos
Feb 25, 2019·Analytical and Bioanalytical Chemistry·Karim VindasElodie Engel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.