DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer

ACS Applied Materials & Interfaces
Thomas BronderMichael J Schöning

Abstract

Miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge favor the semiconductor field-effect platform as one of the most attractive approaches for the development of label-free DNA chips. In this work, a capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with a layer-by-layer prepared, positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was used for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization. The negatively charged probe single-stranded DNA (ssDNA) molecules were electrostatically adsorbed onto the positively charged PAH layer, resulting in a preferentially flat orientation of the ssDNA molecules within the Debye length, thus yielding a reduced charge-screening effect and a higher sensor signal. Each sensor-surface modification step (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), reducing an unspecific adsorption by a blocking agent, incubation with noncomplementary DNA (ncDNA) solution) was monitored by means of capacitance-voltage and constant-capacitance measurements....Continue Reading

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Citations

Nov 23, 2016·IEEE Transactions on Nanobioscience·Rohit ChandYong-Sang Kim
Nov 18, 2017·Scientific Reports·Matti KaistiAri Lehmusvuori
May 23, 2019·The Analyst·Chuanpin ChenTingting Hong
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Dec 18, 2020·Frontiers in Plant Science·Arshak PoghossianMichael J Schöning
May 18, 2018·Analytical Chemistry·Thomas S BronderMichael J Schöning

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