DNA immunization can stimulate florid local inflammation, and the antiviral immunity induced varies depending on injection site

Vaccine
M YokoyamaJ L Whitton

Abstract

DNA immunization is being considered to augment, or even to supplant, more traditional methods of antiviral immunization. Different routes of administration lead to markedly different levels of marker protein expression, but only limited data are available concerning the antiviral responses induced by DNA inoculated by different routes, and their protective efficacy. In this report we evaluate antiviral immunity induced by inoculation of DNA by the intramuscular (i.m.) and intradermal (i.d.) routes, and make three novel observations. First, i.d. immunization is dose-dependent and, although not uniformly successful, can induce very high levels of cytotoxic T lymphocyte (CTL) activity, varying dependent on the vehicle in which the DNA is administered. Second, while antiviral immunity induced by i.m. DNA injection has been demonstrated by many groups, we show herein a marked difference in immunity depending on the muscle injected. Immunity induced by DNA injection of the anterior tibial muscle significantly exceeds that induced following injection of the quadriceps muscle as judged by three criteria, namely CTL induction, decrease in virus titer following nonlethal challenge, and survival following a normally lethal challenge dose...Continue Reading

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Citations

Apr 5, 2001·Journal of Biomedical Science·J HeB L Innis
Apr 17, 2003·Journal of Biotechnology·Zhengrong CuiRussell J Mumper
Apr 9, 1999·Vaccine·J L WhittonD E Hassett
Dec 2, 1999·Vaccine·W W LeitnerN P Restifo
Aug 28, 2001·Veterinary Parasitology·W Kofta, H Wedrychowicz
Apr 13, 1999·Infectious Disease Clinics of North America·J R Haynes
Mar 21, 2002·Immunology and Cell Biology·A K Sharma, G K Khuller
Sep 1, 1999·Rheumatic Diseases Clinics of North America·R K Avery
Dec 26, 2001·The Journal of General Virology·Harrie L GlansbeekPeter J M Rottier
Jul 22, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D G BrockstedtE G Engleman
Aug 12, 2003·Expert Review of Vaccines·Pierre Lemieux
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A La CavaS Albani
Feb 25, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elisa I RivasThomas Stratmann
Apr 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·John R GreenlandNorman L Letvin

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