DNA immunization confers protective immunity on mice challenged intravaginally with herpes simplex virus type 2

Vaccine
Nigel BourneL R Stanberry

Abstract

The immunogenicity and protective efficacy of a nucleic acid vaccine encoding the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) gene under the control of the CMV immediate early gene promoter was examined. Mice immunized three times by intramuscular injection with the vaccine developed an HSV specific IgG but not IgA antibody response detectable in both serum and vaginal secretions. In addition, antigen-specific cellular immune responses were detected in splenic lymphocytes isolated from DNA immunized animals. Immunization reduced virus replication in the genital tract following a lethal intravaginal HSV-2 challenge. Furthermore, symptomatic genital HSV disease was reduced in immunized mice and the animals were completely protected from death. We conclude that a nucleic acid vaccine expressing HSV-1 gD induced both humoral and cell mediated immune responses in mice which proved highly protective against disease following virus challenge.

References

Mar 23, 1990·Science·J A WolffP L Felgner
Jul 20, 1982·The American Journal of Medicine·E R Kern
Dec 1, 1994·Vaccine·D Xu, F Y Liew
Sep 30, 1994·Journal of Immunological Methods·H L VahlsingM Manthorpe
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·E RazM A Yankauckas
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·M SedegahS L Hoffman
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E F FynanH L Robinson

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Citations

Jul 11, 2006·Folia Microbiologica·J Rajcáni, V Durmanová
Feb 12, 1998·Journal of Reproductive Immunology·M B Parr, E L Parr
Apr 9, 1999·Vaccine·D I Bernstein, L R Stanberry
Sep 29, 1999·Veterinary Microbiology·K M RuitenbergJ M Whalley
Apr 13, 1999·Infectious Disease Clinics of North America·P R Krause, S E Straus
Dec 14, 1999·Microbes and Infection·H L Davis, M J McCluskie
May 14, 1999·Pharmaceutical Science & Technology Today·K E ShroffT J Higgins
Aug 21, 2012·Sexually Transmitted Diseases·Frances ValenciaNigel Bourne
Jun 24, 2003·Infection and Immunity·Alejandra V E CapozzoMarina S Palermo
Jun 14, 2000·Journal of Virology·B I Loehr van Drunen Littel-van den Hurk
Apr 9, 1999·Sexually Transmitted Infections·L R Stanberry
Aug 1, 2014·Expert Review of Vaccines·Shane C McAllister, Mark R Schleiss
Aug 8, 2009·Vaccine·Jeffry W PennockNigel Bourne
Mar 6, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Amir GhaemiMassoumeh Ebtekar
May 21, 2005·Reviews in Medical Virology·J RajcániI Rezuchová
Mar 8, 2012·Reviews in Medical Virology·Erin Chung, Jonathan Sen
Dec 8, 2010·Vaccine·Rebecca S TirabassiKejian Yang
Jan 9, 2004·Expert Review of Vaccines·Cheryl A Jones, Anthony L Cunningham
Feb 19, 2003·FEMS Immunology and Medical Microbiology·Marcio O LásaroLuís C S Ferreira
Apr 2, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F X Mbopi-KeouD W G Brown
May 2, 2013·PLoS Pathogens·Leon C W LinDavid C Tscharke
May 22, 2003·Dermatologic Clinics·Mathijs H BrentjensStephen K Tyring
May 13, 2004·Clinical and Diagnostic Laboratory Immunology·L Aurelian

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