PMID: 8978016Jan 1, 1996Paper

DNA immunization of newborn mice with a plasmid-expressing nucleoprotein of influenza virus

Viral Immunology
A BotC Bona

Abstract

Lower responsiveness and higher susceptibility to tolerance are the two main characteristics of neonatal immunity that limit the efficacy of conventional vaccines administered during this period. Based on the fact that DNA immunization of adult organisms is able to generate protective immune responses, we investigated the ability of a plasmid-(NPV1) encoding nucleoprotein (NP) of A/PR8/34 strain of influenza virus to generate a cellular immune response following intramuscular delivery in neonates. Newborn mice immunized with NPV1 plasmid developed significant cytotoxic immunity, comparable to the immune response displayed by adult mice injected with the same dose of plasmid. Furthermore, mice infected with influenza virus 1 month after completion of immunization showed a significant decrease of virus lung titer between day 3 and 7 after challenge, consistent with the protectivity conferred by specific cytotoxic immunity. Thus, mice immunized as neonates with NPV1 plasmid developed a protective cellular immune response, like the adult mice. Therefore, the strategy of DNA immunization may be considered for the purpose of human vaccination to prevent horizontally and vertically transmitted life-threatening infections in infants or...Continue Reading

References

Mar 22, 1996·Science·T ForsthuberP V Lehmann

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Citations

Mar 16, 2000·Developmental and Comparative Immunology·D L Suarez, S Schultz-Cherry
Mar 16, 2000·Developmental and Comparative Immunology·A R Jilbert, I Kotlarski
Mar 24, 1998·Immunology Today·H TigheE Raz
Sep 1, 2000·Advanced Drug Delivery Reviews·J Heppell, H L Davis
May 12, 2001·Vaccine·C A Siegrist
Apr 13, 1999·Infectious Disease Clinics of North America·J R Haynes
Apr 5, 2002·Microbes and Infection·Adrian Bot, Constantin Bona
Dec 14, 1999·Microbes and Infection·H L Davis, M J McCluskie
Oct 9, 1998·Viral Immunology·L Kucerova
Nov 5, 2002·Journal of Virology·Jie ZhangDaniel E Hassett
Dec 31, 1997·The Journal of Clinical Investigation·E ManickanB T Rouse
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C L Brazolot MillanH L Davis
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·X MartinezC A Siegrist
Apr 14, 2000·International Reviews of Immunology·S Fadel, M Sarzotti
Apr 14, 2000·International Reviews of Immunology·A Bot
Sep 2, 2008·Journal of Theoretical Biology·Dobromir T DimitrovGary F McCracken
Sep 25, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Thierry van den BergMichel Bublot
Dec 23, 2006·Journal of Theoretical Biology·Dobromir T DimitrovGary F McCracken
Nov 17, 2020·Frontiers in Immunology·Elizabeth Ann BonneyMercedes Rincon

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