PMID: 9420215Jan 7, 1998Paper

DNA immunization with Japanese encephalitis virus nonstructural protein NS1 elicits protective immunity in mice

Journal of Virology
Y LinH Y Chiang

Abstract

Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is a zoonotic pathogen that is prevalent in some Southeast Asian countries and causes acute encephalitis in humans. To evaluate the potential application of gene immunization to JEV infection, we characterized the immune responses from mice intramuscularly injected with plasmid DNA encoding JEV glycoproteins, including the precursor membrane (prM) plus envelope (E) proteins and the nonstructural protein NS1. When injected with the plasmid expressing prM plus E, 70% of the immunized mice survived after a lethal JEV challenge, whereas when immunized with the plasmid expressing NS1, 90% of the mice survived after a lethal challenge. As a control, the mice immunized with the DNA vector pcDNA3 showed a low level (40%) of protection, suggesting a nonspecific adjuvant effect of the plasmid DNA. Despite having no detectable neutralizing activity, the NS1 immunization elicited a strong antibody response exhibiting cytolytic activity against JEV-infected cells in a complement-dependent manner. By contrast, immunization with a construct expressing a longer NS1 protein (NS1'), containing an extra 60-amino-acid portion from the N terminus of NS2A, failed to protect mice against...Continue Reading

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Citations

Apr 2, 2004·Expert Review of Vaccines·Gwong-Jen J ChangBrent S Davis
Jul 31, 2016·Virology Journal·Meghana RastogiSunit Kumar Singh
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chiou-Feng LinYee-Shin Lin
Mar 22, 2002·Journal of Virology·Shu-Fen WuYi-Ling Lin
Apr 7, 2006·Expert Review of Anti-infective Therapy·Kaushik Bharati, Sudhanshu Vrati
Feb 12, 2009·Journal of Biosciences·Vandana Saxena, Tapan N Dhole
Oct 29, 2013·Human Vaccines & Immunotherapeutics·Sang-Im Yun, Young-Min Lee
Sep 19, 2007·Biotechnology Letters·Cheng-Wen LinWei-June Chen
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Jan 24, 2014·BioMed Research International·Ravi Kant Upadhyay
Aug 5, 2018·Nature Communications·Anzhong LiJianrong Li
Feb 22, 2017·The Journal of General Virology·Yulia V KuzmenkoVadim L Karpov

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