DNA-induced lateral segregation of cationic amphiphiles in lipid bilayer membranes as detected via 2H NMR

Biochemistry
P Mitrakos, P M Macdonald

Abstract

2H NMR spectroscopy was used to investigate the response of specifically choline-deuterated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) to changes in surface electrostatic charge in membranes consisting of mixtures of POPC plus various cationic amphiphiles plus polyadenylic acid (polyA). Three different cationic amphiphiles were investigated: cetyltrimethylammonium bromide (CTAB), dioleoyldimethylaminopropane (DODAP), and 3 beta [N-(N',N'-dimethylaminoethane)carbamoyl]-cholesterol (DC-CHOL). Each of the cationic amphiphiles elicited a concentration-dependent decrease (increase) in the quadrupolar splitting from POPC-alpha-d2 (POPC-beta-d2), as expected for the accumulation of cationic charges at the surface of a lipid bilayer. However, the strength of the response varied with the cationic amphiphile in the order CTAB > DODAP > DC-CHOL. When polyA was added to the cationic amphiphile-containing lipid bilayers, the 2H NMR spectrum consisted of two overlapping Pake patterns, indicating the presence of two lipid domains with different effective surface charges and only slow exchange of lipids between the two domains. There was no evidence of any non-bilayer lipid arrangements. Analysis of the quadrupolar splittings of t...Continue Reading

References

Jan 24, 1991·Nature·P L Felgner, G Rhodes
Aug 1, 1994·Biophysical Journal·P H KonstantS C Harvey

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Citations

Feb 17, 2000·Biochimica Et Biophysica Acta·P Mitrakos, P M Macdonald
May 16, 2000·Chemistry and Physics of Lipids·M Langner, K Kubica
May 16, 2000·Chemistry and Physics of Lipids·M I Angelova, I Tsoneva
May 16, 2000·Solid State Nuclear Magnetic Resonance·P M MacdonaldD Semchyschyn
Oct 15, 2011·Biophysical Chemistry·Petra PullmannováDaniela Uhríková
May 12, 2009·Chemistry and Physics of Lipids·Jean-François LabbéMichèle Auger
May 8, 1999·Biophysical Journal·M KäsbauerT M Bayerl
Feb 7, 2013·Biochimica Et Biophysica Acta·Tânia SilvaMargarida Bastos
Nov 25, 1997·Biochimica Et Biophysica Acta·P A MonnardP Luisi
Nov 14, 1998·Biochimica Et Biophysica Acta·P Mitrakos, P M Macdonald
Dec 18, 2002·Physical Review Letters·Sylvio MayAvinoam Ben-Shaul

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