PMID: 9436259Jan 1, 1997Paper

DNA integrity in human sperm

Teratogenesis, Carcinogenesis, and Mutagenesis
D AndersonM Spanò

Abstract

Since the 1970s there have been conflicting reports of decreasing sperm counts in man and increasing testicular cancer. There is a hypothetical link between apparent adverse trends in several measures of human reproductive health and exposure to endocrine disrupters. Rodent bioassays are not suited for the large-scale screening of such chemicals because of their costs, complexity, and ethical concerns. Various in vitro assays have been used to examine the effects of these chemicals, but none has directly used semen as one of the target tissues in man. The present study has examined in the alkaline Comet assay in human sperm the effect of two estrogens--beta-estradiol and the phytoestrogen daidzein--and 1,2-epoxybutene, a metabolite of 1,3-butadiene, and compared them with the effects of the known reprotoxin, dibromochloropropane, in two fertile and two infertile frozen sperm samples and two fresh fertile samples. While differences were detected in the frozen fertile and infertile samples with flow cytometry, in the Comet assay both frozen and fresh samples exposed to the chemicals in vitro from fertile and infertile men produced similar altered responses by comparison with untreated samples. The integrity of DNA is necessary no...Continue Reading

Citations

May 30, 2006·Cell and Tissue Banking·L GandiniF Dondero
May 15, 2007·Current Opinion in Obstetrics & Gynecology·Mhairi Cl West
Nov 1, 2007·Cell Biology and Toxicology·A BaumgartnerD Anderson
Oct 3, 2018·Frontiers in Endocrinology·Donatella PaoliFrancesco Lombardo
Feb 23, 2010·Systems Biology in Reproductive Medicine·Laiq AhmadOmmia Kalsoom
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde

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