DNA interaction with zinc(II) ions

International Journal of Biological Macromolecules
Lukas NejdlRené Kizek

Abstract

We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored using UV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragment of DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 °C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted into a metal bound DNA (Zn-DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum (190-350 nm) and decrease in the melting temperature (Tm) of Zn-DNA. Spectrophotometric (UV/vis) analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at 200 nm and decrease in absorbance at 251 nm. Application of zinc(II) ions at 5.5 μM concentration caused decrease in Tm for app. 7.5 °C in average in comparison with control (75.5 ± 3 °C). The lowest melting temperature (60.5 ± 2.5 °C) was observed after application of zinc(II) ions at 33 μM concentration. Gel electrophoresis proved significance of Zn(II) in the renaturation of DNA. Samples of Zn-DNA (15 μM DNA+5.5-55 μM Zn(II)) caused significant changes in the renaturation of DNA in comparison with the control, untreated DNA (15 μM DNA).

References

Dec 9, 1988·Nucleic Acids Research·J Blanco, J M Gottesfeld
Sep 28, 2000·Cell·C A Joazeiro, A M Weissman
Dec 13, 2000·Brain Research. Brain Research Reviews·A Takeda
Apr 13, 2001·The Journal of Biological Chemistry·L A Gaither, D J Eide
Mar 7, 2003·Journal of Inorganic Biochemistry·Shawn D WettigJeremy S Lee
Apr 30, 2003·Biophysical Journal·Yi-Tao LongJeremy S Lee
Dec 1, 1962·Journal of Molecular Biology·G FELSENFELD, G SANDEEN
Dec 4, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Haim Tapiero, Kenneth D Tew
Jan 22, 2004·FEMS Yeast Research·Francoise BussereauMonique Bolotin-Fukuhara
Apr 28, 2007·Archives of Biochemistry and Biophysics·John H Laity, Glen K Andrews
Jul 2, 2011·Accounts of Chemical Research·Yandong Yin, Xin Sheng Zhao
May 18, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Michal MasarikRene Kizek
Jul 14, 2012·Metallomics : Integrated Biometal Science·Petr BabulaRene Kizek
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bethany A Buck-KoehntopPeter E Wright
Nov 20, 2012·Current Medicinal Chemistry·V PekarikV Adam

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Citations

Apr 5, 2018·International Journal of Molecular Sciences·Tingxiao QinHongmei Su
May 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nadine WiesmannJuergen Brieger
Jan 4, 2021·Analytical Biochemistry·Corbin J EnglandL Kevin Lewis
Sep 15, 2014·International Journal of Biological Macromolecules·Lukas NejdlRene Kizek

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