DNA ladders can be used to size polyphosphate resolved by polyacrylamide gel electrophoresis

Electrophoresis
Stephanie A SmithJames H Morrissey

Abstract

PAGE is often used to resolve inorganic polyphosphates (polyP), but unfortunately polyP size ladders are not commercially available. Since several dyes that are commonly used to detect nucleic acids in gels also stain polyP, we examined the utility of commercially available DNA size ladders for estimating polyP polymer lengths by gel electrophoresis. Narrow size fractions of polyP were prepared and their polymer lengths were quantified using NMR. Commercially available DNA ladders and these polyP fractions were then subjected to PAGE to determine the relationship between migration of DNA vs polyP, which was found to be: log10 (dsDNA length in bp) = 1.66 × log10 (polyP length in phosphate units) - 1.97. This relationship between DNA and polyP size held for a variety of different polyacrylamide concentrations, indicating that DNA size ladders can readily be employed to estimate polyP polymer lengths by PAGE.

References

Jun 4, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G LeyhausenH C Schröder
Aug 17, 1999·Progress in Molecular and Subcellular Biology·I S KulaevL P Lichko
Aug 17, 1999·Progress in Molecular and Subcellular Biology·H C SchröderW E Müller
Aug 17, 1999·Progress in Molecular and Subcellular Biology·B Lorenz, H C Schröder
Jun 29, 2000·Annual Review of Biochemistry·A KornbergD Ault-Riché
Sep 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lihong WangRichard A Roth
Jan 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephanie A SmithJames H Morrissey
May 12, 2007·The Biochemical Journal·Kyu Yeon HanYong Song Gho
Aug 15, 2008·Biochemistry·Marko TammenkoskiReijo Lahti
Feb 4, 2010·The Journal of Biological Chemistry·Evgeny PavlovAndrey Y Abramov
Apr 6, 2012·International Journal of Hematology·James H Morrissey
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
May 10, 2013·PLoS Pathogens·Silvia N J Moreno, Roberto Docampo
Mar 17, 2015·Molecular Cell·Cristina AzevedoAdolfo Saiardi

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Citations

Mar 20, 2020·Biotechnology and Bioengineering·Jonas Johannes ChristLars Mathias Blank
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Feb 27, 2021·Trends in Microbiology·Marvin Q Bowlin, Michael J Gray
Feb 11, 2020·Analytical Chemistry·Jonas Johannes ChristLars M Blank
Oct 8, 2021·Frontiers in Plant Science·Cuc Thi Nguyen, Katsuharu Saito

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