DNA-Mediated Nanoscale Metal-Organic Frameworks for Ultrasensitive Photoelectrochemical Enzyme-Free Immunoassay

Analytical Chemistry
Guang-Yao ZhangXue-Ji Zhang

Abstract

A novel enzyme-free photoelectrochemical (PEC) immunoassay was developed for the ultrasensitive detection of prostate specific antigen (PSA) based on the DNA-mediated nanoscale zirconium-porphyrin MOFs (NMOFs). By virtue of the intrinsic coordination between unsaturated zirconium sites of the NMOFs frameworks and phosphonate groups, the 5'-phosphorylared ss-DNA-tagged antibody (Ab-DNA) conjugate with a consecutive stretch of guanines as a spacer could be loaded on the NMOFs easily, obtaining a novel type of Ab-DNA-functionalized NMOFs complex. Additionally, as a photocathode PEC active nanomaterial, NMOFs exhibited a significant enhanced photocurrent response with the presence of dopamine under oxygen-containing aqueous media at -0.3 V (vs Ag/AgCl). Furthermore, with the aid of the electrochemical grafting of polyamidoamine (PAMAM) dendrimers functionalized interface, the novel type of Ab-DNA-NMOFs further served as a PEC signal nanoprobe for the ultrasensitive PSA immunoassay. Under optimal conditions, the corresponding immunosensor possessed a wide calibration range of 1 pg mL-1 to 10 ng mL-1 and a limit of detection (LOD) of 0.2 pg mL-1. This present work demonstrated the promising application of DNA-mediated NMOFs in develo...Continue Reading

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Citations

Apr 10, 2020·Biotechnology Journal·Liping DuChunsheng Wu
Feb 18, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Fen WuQian Shi
Jan 31, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sadia AfreenJun-Jie Zhu
Jan 29, 2020·Angewandte Chemie·Jiajie ChenStefan Kaskel
Mar 9, 2021·Coordination Chemistry Reviews·Xuan ZhangOmar K Farha
Jul 14, 2020·ACS Applied Materials & Interfaces·Xiang-Jing KongJian-Rong Li
May 19, 2020·Analytical Chemistry·Feng-Zao ChenHong-Yuan Chen

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