Mar 1, 1979

DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells

Proceedings of the National Academy of Sciences of the United States of America
M WiglerL Chasin

Abstract

In this report, we demonstrate the feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by means of DNA-mediated gene transfer. Transformation was effected by using unfractionated high molecular weight genomic DNA from Chinese hamster, human, and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was between 1 and 10 colonies per 10(6) cells per 20 microgram of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into two classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.

  • References11
  • Citations690

References

  • References11
  • Citations690

Citations

Mentioned in this Paper

Transformation, Genetic
Adenine Phosphoribosyltransferase
Pentosyltransferase
APRT
Salmo salmo
Recombination Joint
HeLa Cells
DNA Restriction Enzymes
Cricetus
Clone Cells

About this Paper

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