DNA melting and genotoxicity induced by silver nanoparticles and graphene

Chemical Research in Toxicology
Angela IvaskPu Chun Ke

Abstract

We have revealed a connection between DNA-nanoparticle (NP) binding and in vitro DNA damage induced by citrate- and branched polyethylenimine-coated silver nanoparticles (c-AgNPs and b-AgNPs) as well as graphene oxide (GO) nanosheets. All three types of nanostructures triggered an early onset of DNA melting, where the extent of the melting point shift depends upon both the type and concentration of the NPs. Specifically, at a DNA/NP weight ratio of 1.1/1, the melting temperature of lambda DNA dropped from 94 °C down to 76 °C, 60 °C, and room temperature for GO, c-AgNPs and b-AgNPs, respectively. Consistently, dynamic light scattering revealed that the largest changes in DNA hydrodynamic size were also associated with the binding of b-AgNPs. Upon introduction to cells, b-AgNPs also exhibited the highest cytotoxicity, at the half-maximal inhibitory (IC50) concentrations of 3.2, 2.9, and 5.2 mg/L for B and T-lymphocyte cell lines and primary lymphocytes, compared to the values of 13.4, 12.2, and 12.5 mg/L for c-AgNPs and 331, 251, and 120 mg/L for GO nanosheets, respectively. At cytotoxic concentrations, all NPs elicited elevated genotoxicities via the increased number of micronuclei in the lymphocyte cells. However, b-AgNPs also ...Continue Reading

References

Nov 17, 2006·Nature·Andrew D MaynardDavid B Warheit
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tommy CedervallSara Linse
Jun 5, 2007·Nature Protocols·Michael Fenech
Mar 19, 2008·Journal of Colloid and Interface Science·Soumen BasuTarasankar Pal
Oct 22, 2008·Toxicology and Applied Pharmacology·Maqusood AhamedYiling Hong
Feb 25, 2009·ACS Nano·P V AshaRaniSuresh Valiyaveettil
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Sep 8, 2009·Mutation Research·Jasminka MrdanovićAleksandar Djordjevic
Dec 7, 2010·Toxicology Letters·Yanli ChangHaifang Wang
Dec 21, 2010·Nature Nanotechnology·Zhou J DengRodney F Minchin
Jan 12, 2011·Environmental Science & Technology·Bernd NowackMurray Height
Feb 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Marissa WuJuewen Liu
Nov 1, 2006·Environmental Health and Preventive Medicine·Yasuharu Niwa, Naoharu Iwai
May 3, 2011·PloS One·Saliha AkcaHenk W Ch Postma
Aug 25, 2011·ACS Nano·Martin LundqvistKenneth Dawson
Nov 17, 2011·Toxicology·Nana AsareGunnar Brunborg
Aug 7, 2012·Biomaterials·Omid AkhavanAlireza Akhavan
Sep 11, 2014·Accounts of Chemical Research·Nadrian C SeemanHao Yan
Dec 3, 2014·Physical Chemistry Chemical Physics : PCCP·Bo WangPu Chun Ke

❮ Previous
Next ❯

Citations

Mar 13, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Abhilash SasidharanManzoor Koyakutty
Jul 19, 2016·Chemical Research in Toxicology·Joel A Pedersen, Agnes Kane
Oct 23, 2016·Journal of Biomedical Materials Research. Part a·Iêda Maria Martinez PainoValtencir Zucolotto
Nov 2, 2016·Particle and Fibre Toxicology·Lingling OuLongquan Shao
Feb 7, 2020·Nanomaterials·Adriana Rodriguez-GarrausAdela López de Cerain
May 26, 2017·British Journal of Neurosurgery·Aaron TanKeyoumars Ashkan
Aug 21, 2019·Oxidative Medicine and Cellular Longevity·Lucía M Valenzuela-SalasNina Bogdanchikova
Mar 21, 2020·Mutation Research. Reviews in Mutation Research·Hadi SamadianAmirhossein Ahmadi
Jul 27, 2021·Advanced Healthcare Materials·Huiliang CaoKlaus D Jandt
Jun 18, 2015·Biomacromolecules·Federica PaladiniLuigi Ambrosio

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.