DNA-membrane complex: macromolecular content and stimulation of enzymatic activity by polyadenylic acid

Science
W Firshein, R G Gillmor

Abstract

A DNA-cell membrane complex has been isolated from cell suspensions of virulent pneumococci by sarcosyl lysis followed by centrifugation on a sucrose gradient. When polyadenylic acid plus the eight naturally occurring deoxyribonucleosides and deoxyribonucleotides are added to cell suspensions, the percentage of total DNA in the complex increases with incubation time. This increase is not observed in unsupplemented cell suspensions. However, the percentages of RNA, protein, and phospholipid do not increase with incubation time in either supplemented or control complexes. A variety of deoxyribonucleotide kinases and the DNA polymerase are also detected in the DNA-membrane complex, and their specific activities are greater in complexes extracted from supplemented cell suspensions than in those extracted from controls.

References

Oct 15, 1975·Biochimica Et Biophysica Acta·M C Gomez-Eichelmann, F Bastarrachea
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D J LeBlanc, M F Singer
Mar 9, 2012·Clinical & Developmental Immunology·Vidyalakshmi ChandramohanDarell D Bigner
Oct 14, 1972·Journal of Molecular Biology·W Firshein
Mar 7, 1973·Nature: New Biology·A A InfanteW Firshein

Related Concepts

Pathologic Cytolysis
Macromolecular Compounds
Centrifugation, Density Gradient
Bacterial Proteins
Complex (molecular entity)
Deoxyribonucleosides
Polynucleotides
Centrifugation
Alkaline DNase
Phospholipids

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