DNA metabarcoding of spiders, insects, and springtails for exploring potential linkage between above- and below-ground food webs

Zoological Letters
Hirokazu Toju, Yuki G Baba

Abstract

Understanding feedback between above- and below-ground processes of biological communities is a key to the effective management of natural and agricultural ecosystems. However, as above- and below-ground food webs are often studied separately, our knowledge of material flow and community dynamics in terrestrial ecosystems remains limited. We developed a high-throughput sequencing method for examining how spiders link above- and below-ground food webs as generalist predators. To overcome problems related to DNA-barcoding-based analyses of arthropod-arthropod interactions, we designed spider-specific blocking primers and Hexapoda-specific primers for the selective PCR amplification of Hexapoda prey sequences from spider samples. By applying the new DNA metabarcoding framework to spider samples collected in a temperate secondary forest in Japan, we explored the structure of a food web involving 15 spider species and various taxonomic groups of Hexapoda prey. These results support the hypothesis that multiple spider species in a community can prey on both above- and below-ground prey species, potentially coupling above- and below-ground food-web dynamics. The PCR primers and metabarcoding pipeline described in this study are expect...Continue Reading

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Citations

Sep 19, 2018·Molecular Ecology Resources·Daniel MarquinaFredrik Ronquist
Jun 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yasunori IchihashiNaoto Nihei
Nov 24, 2020·Ecology·David R Hemprich-BennettElizabeth L Clare
Mar 31, 2021·The Journal of Animal Ecology·Matthew A McCary, Oswald J Schmitz
Jun 19, 2021·Ecology and Evolution·Diego Sierra RamírezCarlos Fernando Prada Quiroga

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Datasets Mentioned

BETA
PRJDB5193

Methods Mentioned

BETA
PCR
Illumina sequencing
genotyping

Software Mentioned

MiSeq Reporter
MAFFT
VSEARCH
perl
MiSeq
vegan
bcl2fastq
R
bipartite
Claident

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