DNA-methylation alterations and exchanges during in vitro cellular differentiation in rose (Rosa hybrida L.)

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Mingliang XuSchuyler S Korban

Abstract

DNA-methylation profiles of leaf tissues of Rosa hybrida cv. Carefree Beauty collected from in vivo-grown greenhouse plants, in vitro-grown proliferating shoots at different passages, regenerants of embryogenic callus, regenerants of organogenic callus, as well as calli from undifferentiated callus (UC), embryogenic callus, and organogenic callus were investigated using an amplified fragment-length polymorphism (AFLP)-based detection technique. Three types of AFLP bands were recovered. Type I bands were observed with both isoschizomers Msp and HpaII, while type II and type III bands were observed only with MspI and HpaII, respectively. Sequence analysis of the three types of AFLP bands revealed that a nonmethylated MspI/HpaII-recognition site 5'-CCGG-3' resulted in a type I band, while an inner 5-methylcytosine generated most type II and type III bands. About 40% of inner and 20% of outer cytosines in 5'-CCGG-3' sequences were fully methylated, and only a few hemimethylated outer cytosines were observed. Changes in types of AFLP bands among different tissues were frequently observed, including appearance and disappearance of type I, II, and III AFLP bands, as well as exchanges between either type I and type II or type I and typ...Continue Reading

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Citations

Oct 16, 2007·Animal Biotechnology·Qing XuYing Yu
May 28, 2011·Journal of Experimental Botany·Célia Miguel, Liliana Marum
Jul 17, 2008·BMC Plant Biology·Paul SchellenbaumBernard Walter
Feb 2, 2011·PloS One·Chun YangZhihui Zhao
Jun 29, 2011·Genetics and Molecular Research : GMR·C EvrenselN Gozukirmizi
Jan 8, 2014·BMC Genetics·Jaroslav Fulneček, Aleš Kovařík
Mar 8, 2007·Biochimica Et Biophysica Acta·Mary Gehring, Steven Henikoff
Sep 9, 2015·Frontiers in Plant Science·Clelia De-la-PeñaVíctor M Loyola-Vargas
Dec 15, 2016·Frontiers in Plant Science·Jihua XuStephen J Robinson
Oct 20, 2018·International Journal of Molecular Sciences·Pedro Osorio-MontalvoClelia De-la-Peña
Feb 23, 2019·Frontiers in Plant Science·Hugo A Méndez-HernándezVíctor M Loyola-Vargas
Feb 13, 2020·Plant Molecular Biology·Renata Orłowska, Piotr Tomasz Bednarek
Jan 1, 2016·3 Biotech·Hare KrishnaRadha Kishan Sadh
Feb 1, 2019·Current Opinion in Plant Biology·Kaoru SugimotoSachihiro Matsunaga

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