DNA methylation analysis of cancer-related genes in oral epithelial cells of healthy smokers

Archives of Oral Biology
Sabrina Rocha Luna De OliveiraNaila Francis Paulo De Oliveira

Abstract

The aim of this study was to investigate the smoking habit influence on DNA methylation status in the promoters of the cancer related-genes MLH1, hTERT and TP53 in oral epithelial cells of healthy subjects. DNA methylation analysis was performed using methylation-sensitive restriction enzymes (MSRE) in oral epithelial cells from non-smokers, smokers and ex-smokers. The investigated CpG dinucleotides located at HhaI and HpaII sites in the MLH1 gene promoter were observed to be fully methylated in the majority of DNA samples from the smoker group and statistical differences were found between non-smokers and smokers and between smokers and ex-smokers (p<0.05). The same was observed in the hTERT gene promoter at HhaI sites (p<0.05) and for HpaII sites the unmethylated condition was more frequent in smokers in comparison to non-smokers (p<0.05). For TP53, no differences were found among groups (p>0.05), with the fully methylated condition found to be a common event in healthy oral epithelial cells. We conclude that smoking may induce changes in DNA methylation status in cancer-related genes of oral epithelial cells and that the cessation of smoking is capable of reversing this process. Based on our data, we suggest that DNA methyla...Continue Reading

References

May 1, 1989·Molecular and Cellular Biology·S P Tuck, L Crawford
May 1, 1986·Molecular and Cellular Biology·P Lamb, L Crawford
Nov 7, 1999·The American Journal of Pathology·M EstellerJ G Herman
Oct 16, 2002·Oncogene·Ying Chuan HuSteven A Ahrendt
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Muthusamy ViswanathanGovindaswamy Shanmugam
Oct 7, 2003·Annual Review of Microbiology·Mark J Schofield, Peggy Hsieh
Nov 26, 2003·Molecular Carcinogenesis·Xabier AgirreJosé L Vizmanos
Nov 16, 2004·Biochemical and Biophysical Research Communications·Isabelle Guilleret, Jean Benhattar
Apr 13, 2006·Chemical Research in Toxicology·Victoria ValinluckLawrence C Sowers
May 13, 2006·Endocrinology·Matthew D Anway, Michael K Skinner
Nov 30, 2006·PLoS Medicine·Colin D Mathers, Dejan Loncar
Dec 5, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ruo-Kai LinYi-Ching Wang
Oct 30, 2007·Epigenetics : Official Journal of the DNA Methylation Society·Ko HashimotoHelmtrud I Roach
Nov 6, 2007·Brazilian Dental Journal·Marisi Aidar, Sergio Roberto Peres Line
Mar 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ekaterina BaryshnikovaMassimo Roncalli
Oct 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·R SattaA Guidotti
Feb 24, 2009·Nature Reviews. Molecular Cell Biology·Aline V ProbstGeneviève Almouzni
Feb 27, 2009·Future Oncology·Julia A Woolgar, Gillian L Hall
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Anna CsiszarZoltan Ungvari
Jan 19, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Ramin RadpourXiao Yan Zhong
May 26, 2010·Aging Cell·Reid F ThompsonNir Barzilai
Nov 16, 2010·Oral Oncology·I González-RamírezC M García-Cuellar
Jun 9, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Raghu RadhakrishnanKapaettu Satyamoorthy
Aug 19, 2011·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Markênia Kélia Santos AlvesSilvia Helena Barem Rabenhorst
Oct 21, 2011·Brazilian Dental Journal·Paula Rocha MoreiraRicardo Santiago Gomez
Mar 3, 2012·Gene·Michael DanielTrygve O Tollefsbol
Oct 3, 2012·PloS One·Siriporn WangsriApiwat Mutirangura

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Citations

Jan 25, 2018·Anais Brasileiros De Dermatologia·Mikaelly Batista da SilvaNaila Francis Paulo de Oliveira
Sep 18, 2020·The Journal of International Medical Research·Gabriela-Emilia AvramCatalin Marian
May 11, 2018·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Steven R H BeachRobert A Philibert
Apr 8, 2019·Biological Research·Raquel Patrícia Ataíde LimaMaria José de Carvalho Costa
Oct 29, 2017·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Nadja NikolicJelena Milasin

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