Epigenetic marks are heritable, influenced by the environment, direct the maturation of T lymphocytes, and in mice enhance the development of allergic airway disease. Thus it is important to define epigenetic alterations in asthmatic populations. We hypothesize that epigenetic alterations in circulating PBMCs are associated with allergic asthma. We compared DNA methylation patterns and gene expression in inner-city children with persistent atopic asthma versus healthy control subjects by using DNA and RNA from PBMCs. Results were validated in an independent population of asthmatic patients. Comparing asthmatic patients (n = 97) with control subjects (n = 97), we identified 81 regions that were differentially methylated. Several immune genes were hypomethylated in asthma, including IL13, RUNX3, and specific genes relevant to T lymphocytes (TIGIT). Among asthmatic patients, 11 differentially methylated regions were associated with higher serum IgE concentrations, and 16 were associated with percent predicted FEV1. Hypomethylated and hypermethylated regions were associated with increased and decreased gene expression, respectively (P < 6 × 10(-12) for asthma and P < .01 for IgE). We further explored the relationship between DNA me...Continue Reading
Associated Clinical Trials
DNA methylation changes at human Th2 cytokine genes coincide with DNase I hypersensitive site formation during CD4(+) T cell differentiation
DNA methylation disturbances as novel therapeutic target in lung cancer: preclinical and clinical results
Accelerated chemokine receptor 7-mediated dendritic cell migration in Runx3 knockout mice and the spontaneous development of asthma-like disease
Linear models and empirical bayes methods for assessing differential expression in microarray experiments
The human IL-13 locus in neonatal CD4+ T cells is refractory to the acquisition of a repressive chromatin architecture
CpG methylation patterns in the IFNgamma promoter in naive T cells: variations during Th1 and Th2 differentiation and between atopics and non-atopics
Transcription factors T-bet and Runx3 cooperate to activate Ifng and silence Il4 in T helper type 1 cells
The human colon cancer methylome shows similar hypo- and hypermethylation at conserved tissue-specific CpG island shores.
A Bayesian framework to account for complex non-genetic factors in gene expression levels greatly increases power in eQTL studies.
DNA methylation in the arginase-nitric oxide synthase pathway is associated with exhaled nitric oxide in children with asthma.
Epigenetic regulation in murine offspring as a novel mechanism for transmaternal asthma protection induced by microbes.
Krüppel-like factor 6 regulates transforming growth factor-β gene expression during human respiratory syncytial virus infection.
Quantitative cross-validation and content analysis of the 450k DNA methylation array from Illumina, Inc.
Complete pipeline for Infinium(®) Human Methylation 450K BeadChip data processing using subset quantile normalization for accurate DNA methylation estimation
SWAN: Subset-quantile within array normalization for illumina infinium HumanMethylation450 BeadChips.
Transactivation of inducible nitric oxide synthase gene by Kruppel-like factor 6 regulates apoptosis during influenza A virus infection.
Hormone Replacement Therapy Associated White Blood Cell DNA Methylation and Gene Expression are Associated With Within-Pair Differences of Body Adiposity and Bone Mass
Methylomic markers of persistent childhood asthma: a longitudinal study of asthma-discordant monozygotic twins
Comparative DNA methylation analysis to decipher common and cell type-specific patterns among multiple cell types
DNA methylation loci associated with atopy and high serum IgE: a genome-wide application of recursive Random Forest feature selection
Endotypes of allergic diseases and asthma: An important step in building blocks for the future of precision medicine
An American Thoracic Society/National Heart, Lung, and Blood Institute Workshop Report: Addressing Respiratory Health Equality in the United States
Allergic asthma: an overview of metabolomic strategies leading to the identification of biomarkers in the field
Neonatal Immune State Is Influenced by Maternal Allergic Rhinitis and Associated With Regulatory T cells.
Dust mite allergen-specific immunotherapy increases IL4 DNA methylation and induces Der p-specific T cell tolerance in children with allergic asthma
Transgenerational transmission of asthma risk after exposure to environmental particles during pregnancy
The use of pharmacogenomics, epigenomics, and transcriptomics to improve childhood asthma management: Where do we stand?
Maternal Exposure of BALB/c Mice to Indoor NO2 and Allergic Asthma Syndrome in Offspring at Adulthood with Evaluation of DNA Methylation Associated Th2 Polarization
Gata3 hypermethylation and Foxp3 hypomethylation are associated with sustained protection and bystander effect following epicutaneous immunotherapy in peanut-sensitized mice
Nasal DNA methylation differentiates corticosteroid treatment response in pediatric asthma: A pilot study.
Differentially methylated DNA regions in early childhood wheezing: An epigenome-wide study using saliva
Promoting respiratory public health through epigenetics research: an ERS Environment Health Committee workshop report
Frequency of heavy vehicle traffic and association with DNA methylation at age 18 years in a subset of the Isle of Wight birth cohort
Newborn DNA-methylation, childhood lung function, and the risks of asthma and COPD across the life course.
Prenatal exposure to tobacco smoke sex dependently influences methylation and mRNA levels of the Igf axis in lungs of mouse offspring
Insights from deconvolution of cell subtype proportions enhance the interpretation of functional genomic data
Epigenetic outlier profiles in depression: A genome-wide DNA methylation analysis of monozygotic twins
Comparing DNA methylation profiles across different tissues associated with the diagnosis of pediatric asthma
Cancer Biology: Molecular Imaging
Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.
Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.
Allergy and Asthma
Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.
B cells: Gene Expression
B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.
This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.