DNA methylation-dependent epigenetic regulation of dimethylarginine dimethylaminohydrolase 2 gene in trophoblast cell lineage

The Journal of Biological Chemistry
Junko TomikawaKunio Shiota

Abstract

Trophoblast cell lineage is established through the first cellular differentiation in mammalian embryogenesis, and its developmental potential is restricted to the extraembryonic tissues contributing solely to the placenta. Several lines of evidence suggest a relative lack of importance of DNA methylation in gene regulation in the extraembryonic tissues when compared with embryonic ones. Here we analyzed the dynamics of epigenetic status in the upstream region of mouse Ddah2 gene, which was found to be specifically repressed in a stem cell population of trophoblast cell lineage. We found a tissue-dependent differentially methylated region in the regulatory region of the Ddah2 gene. This region was hypermethylated in trophoblast stem cells and was hypomethylated in differentiated cells both in vivo and in vitro. This change was well correlated with Ddah2 expression. In addition, in vitro methylation confined to the differentially methylated region was sufficient to repress promoter activity in the reporter assay. Furthermore, a repressive pattern of histone modifications was formed around the differentially methylated region in undifferentiated trophoblast stem cells with repressed Ddah2. Our data suggest that DNA methylation-me...Continue Reading

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Citations

Dec 24, 2011·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Koji HayakawaKunio Shiota
May 1, 2010·Zygote : the Biology of Gametes and Early Embryos·Lleretny Rodríguez-AlvarezFidel Ovidio Castro
Apr 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Junko TomikawaHiroko Tsukamura
Aug 11, 2010·Hypertension·Zaiming LuoChristopher S Wilcox
May 25, 2011·The Journal of Reproduction and Development·Shun SatoKunio Shiota
Jun 4, 2011·The Journal of Reproduction and Development·Koichiro NishinoKunio Shiota
Jun 5, 2010·PLoS Genetics·Elke GrönnigerFrank Lyko
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Leann BlombergChristoph Viebahn
May 15, 2012·Reproductive Biomedicine Online·Peter J Rugg-Gunn
Mar 14, 2009·Differentiation; Research in Biological Diversity·Katja LinherJulang Li
Mar 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Naoko HattoriKunio Shiota
Dec 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masako SuzukiKunio Shiota
Nov 1, 2007·BJOG : an International Journal of Obstetrics and Gynaecology·T M NafeeK M K Ismail
Jan 11, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kohta IkegamiKunio Shiota
Jul 16, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroki MuramotoKunio Shiota
Mar 8, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Momo O NakanishiSatoshi Tanaka
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Nov 27, 2018·Epigenetics : Official Journal of the DNA Methylation Society·Teena K J B GamageJoanna L James
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Oct 16, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Fredrik PalmChristopher S Wilcox
May 21, 2021·Cellular and Molecular Neurobiology·Alena A KozlovaNadine Bernhardt

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