DNA Methylation Epigenetically Regulates Gene Expression in Burkholderia cenocepacia and Controls Biofilm Formation, Cell Aggregation, and Motility.

MSphere
Ian VandenbusscheTom Coenye

Abstract

Respiratory tract infections by the opportunistic pathogen Burkholderia cenocepacia often lead to severe lung damage in cystic fibrosis (CF) patients. New insights in how to tackle these infections might emerge from the field of epigenetics, as DNA methylation is an important regulator of gene expression. The present study focused on two DNA methyltransferases (MTases) in B. cenocepacia strains J2315 and K56-2 and their role in regulating gene expression. In silico predicted DNA MTase genes BCAL3494 and BCAM0992 were deleted in both strains, and the phenotypes of the resulting deletion mutants were studied: deletion mutant ΔBCAL3494 showed changes in biofilm structure and cell aggregation, while ΔBCAM0992 was less motile. B. cenocepacia wild-type cultures treated with sinefungin, a known DNA MTase inhibitor, exhibited the same phenotype as DNA MTase deletion mutants. Single-molecule real-time sequencing was used to characterize the methylome of B. cenocepacia, including methylation at the origin of replication, and motifs CACAG and GTWWAC were identified as targets of BCAL3494 and BCAM0992, respectively. All genes with methylated motifs in their putative promoter region were identified, and qPCR experiments showed an upregulati...Continue Reading

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Citations

Jul 28, 2021·Annual Review of Microbiology·Brian P Anton, Richard J Roberts
Nov 11, 2021·Applied Microbiology and Biotechnology·Viola Camilla ScoffoneSilvia Buroni

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Methods Mentioned

BETA
flow cytometry
motility assay
PCR
fluorescence microscopy

Software Mentioned

Artemis Genome Browser Annotation Tool
Virtual
ImageJ
Sanger
Bio
Base Modification and Motif Analysis application ( SMRT Link 0
CLC Workbench Genomics
Virtual Footprint
SMRT Link
Rad CFX Manager

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