DNA Methylation Fluctuation Induced by Virus Infection Differs between MD-resistant and -susceptible Chickens.

Frontiers in Genetics
Juan LuoJiuzhou Song

Abstract

Marek's disease (MD) is a lymphoproliferative disease induced by Marek's disease virus (MDV) infection. To augment vaccination measures in MD control, host genetic resistant to MD becomes obviously more and more important. To elucidate the mechanism of MD-resistance, most of researches were focused on the genetic differences between resistant and susceptible chickens. However, epigenetic features between MD resistant and susceptible chickens are poorly characterized. Using bisulfite pyrosequencing method, we found some candidate genes have higher promoter methylation in the MD-susceptible (L7(2)) chickens than in the MD-resistant (L6(3)) chickens. The hypermethylated genes, involved in cellular component organization, responding to stimulus, cell adhesion, and immune system process, may play important role in susceptibility to disease by deregulation of these genes. MDV infection induced the expression changes of all three methyltransferases genes (DNMT1, DNMT3a, and DNMT3b) in both lines of chickens. The DNMT1 was up-regulated in L7(2), whereas the DNMT3b was down-regulated in L6(3) at 21 dpi. Interestingly, a dynamic change of promoter methylation was observed during MDV life cycle. Some genes, including HDAC9, GH, STAT1, CII...Continue Reading

Citations

Jan 7, 2014·PloS One·Renáta TóthZoltán Zádori
Sep 17, 2013·Virus Research·David SibonEric Wattel
Mar 30, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Fei TianJiuzhou Song
Mar 20, 2015·Frontiers in Genetics·Silvia C GalvanFélix Recillas-Targa
Jun 30, 2019·International Journal of Molecular Sciences·Qin ChuJiuzhou Song
Jun 1, 2021·Poultry Science·Marek BednarczykEwa Grochowska

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

PANTHER
Primer3
PSQ Assay Design

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