DNA methylation in human papillomavirus-infected cervical cells is elevated in high-grade squamous intraepithelial lesions and cancer

Journal of Gynecologic Oncology
Mi-Kyung KimTae-Jin Kim

Abstract

DNA methylation has been shown to be a potential biomarker for early cancer detection. The aim of this study was to evaluate DNA methylation profiles according to liquid-based Pap (LBP) test results and to assess their diagnostic value in a Korean population. A total of 205 patients with various Papanicolaou test results were enrolled to this study (negative, 26; atypical squamous cells of undetermined significance, 39; low grade squamous intraepithelial lesion, 44; high grade squamous intraepithelial lesion (HSIL), 48; and cancer, 48). DNA methylation analysis of four genes, ADCYAP1, PAX1, MAL, and CADM1, was performed on residual cervical cells from LBP samples using a quantitative bisulfite pyrosequencing method. To evaluate the diagnostic performance of the four methylated genes for cancer detection, receiver operating characteristic (ROC) curves were drawn. Sensitivities and specificities were also tested at cutoffs determined from the ROC curves. Cervical cancer cells showed dramatically increased methylation levels for the four genes analyzed. ADCYAP1 and PAX1 also trended toward elevated methylation levels in HSIL samples, although the levels were much lower than those in cancer cells. The sensitivities of methylated AD...Continue Reading

References

Dec 13, 2006·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·M A QuinnS Pecorelli
Oct 19, 2007·The New England Journal of Medicine·Marie-Hélène MayrandUNKNOWN Canadian Cervical Cancer Screening Trial Study Group
Nov 7, 2008·Methods in Molecular Biology·Emelyne DejeuxJörg Tost
Mar 11, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Albertus T HesselinkPeter J F Snijders
Apr 11, 2012·International Journal of Cancer. Journal International Du Cancer·Nicolas WentzensenSophia S Wang
Sep 5, 2012·Nature Reviews. Genetics·Holger Heyn, Manel Esteller
Oct 25, 2012·Journal of Gynecologic Oncology·Jonathan D BooneWarner K Huh
Jan 25, 2013·Journal of Gynecologic Oncology·Won-Chul LeeJong Sup Park
Mar 5, 2013·International Journal of Cancer. Journal International Du Cancer·Mariska BierkensRenske D M Steenbergen
Oct 25, 2013·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Kyu-Won JungJin-Soo Lee
May 23, 2014·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Yuen-Yee KanCheng-I Liao
Jun 26, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Lise M A De StrooperDaniëlle A M Heideman
Nov 1, 2006·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·M A QuinnS Pecorelli

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Citations

Jul 19, 2018·ESMO Open·Peter A van DamSteven Van Laere
Apr 7, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Maria PanagopoulouEkaterini Chatzaki
Jun 27, 2019·Cells·Patti OlusolaSantanu Dasgupta

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Methods Mentioned

BETA
biopsy
biopsies
genotyping
Chip
PCR
Assay
methylation profiling

Software Mentioned

MedCalc
PyroMark Assay Design

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