PMID: 15383570Sep 24, 2004Paper

DNA methylation is a nonredundant repressor of the Th2 effector program

The Journal of Immunology : Official Journal of the American Association of Immunologists
Karen W Makar, Christopher B Wilson

Abstract

The extent to which DNA methylation contributes to proper regulation of murine T cell effector function is unclear. In this study, we show that in the absence of the maintenance DNA methyltransferase Dnmt1, silencing of IL-4, IL-5, IL-13, and IL-10 in CD8 T cells was abolished, and expression of these Th2 cytokines increased as much as 1000-fold compared with that of control CD8 T cells. Th2 cytokine expression also increased in Dnmt1(-/-) CD4 T cells, but the increase ( approximately 20-40-fold for IL-4 and IL-10, </=5-fold for IL-5 and IL-13) was less than for CD8 T cells. As a result, both Dnmt1(-/-) CD4 and CD8 T cells expressed high and comparable amounts of Th2 cytokines. Loss of Dnmt1 had more subtle effects on IL-2 (</=5-fold increase) and IFN-gamma ( approximately 5-10-fold increase) expression and did not affect the normal bias for greater IL-2 expression by CD4 T cells and greater IFN-gamma expression by CD8 T cells, nor the exclusive expression of perforin and granzyme B by the CD8 T cells. These results indicate that Dnmt1 and DNA methylation are necessary to prevent cell autonomous Th2 cytokine expression in CD8 T cells but are not essential for maintaining proper T cell subset-specific expression of Th1 or CTL ef...Continue Reading

References

Sep 5, 1998·Immunity·J J BirdS L Reiner
Mar 10, 2001·Cold Spring Harbor Symposia on Quantitative Biology·I C HoL H Glimcher
Aug 2, 2002·Molecular Cell·Anne S HutchinsSteven L Reiner
Dec 4, 2002·Nature Reviews. Immunology·Kenneth M Murphy, Steven L Reiner
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qianjin LuBruce Richardson
Nov 5, 2003·Nature Immunology·Karen W MakarChristopher B Wilson

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Citations

Jan 20, 2009·Nature Reviews. Immunology·Christopher B WilsonMasayuki Sekimata
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew D Wells
Jul 25, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christopher J GamperJonathan D Powell
Mar 4, 2011·Cellular and Molecular Life Sciences : CMLS·Leigh Matthew MarshHarald Renz
Dec 16, 2010·Circulation. Cardiovascular Genetics·Andrea BaccarelliEmelia J Benjamin
Oct 12, 2016·BMC Medical Genetics·Ramon Bossardi RamosPoli Mara Spritzer
Oct 30, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Bénédicte L TremblayMarie-Claude Vohl
Apr 6, 2005·Human Molecular Genetics·Steven L Reiner
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne S HutchinsSteven L Reiner
Jul 15, 2005·World Journal of Gastroenterology : WJG·Sheng LiJin-Ming Yu
Aug 22, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Amanda J MacFarlaneFraser W Scott
Apr 25, 2008·Autoimmunity·Amr H Sawalha
Jun 19, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jonathan G MoggsIan Kimber
Feb 13, 2013·Nature Reviews. Genetics·Zachary D Smith, Alexander Meissner
Jan 15, 2015·Immunology and Cell Biology·Frann Antignano, Colby Zaph
Dec 11, 2013·Archives of Dermatological Research·Geon Tae ParkTae-Yoon Kim
Dec 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Qing YuMark H Kaplan
Feb 24, 2015·Frontiers in Immunology·J Scott Hale, Rafi Ahmed
Jan 20, 2018·Oxidative Medicine and Cellular Longevity·Gang LiuYulong Yin
Mar 6, 2018·Cellular & Molecular Immunology·Peng Zhang, Qianjin Lu
Jul 2, 2020·American Journal of Respiratory Cell and Molecular Biology·Andrew D PriggeKaren M Ridge
Nov 2, 2004·Nature Immunology·K Mark AnselAnjana Rao
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rajan M ThomasAndrew D Wells
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazuhisa AokiShoichiro Miyatake
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shawn RoseBecky Adkins
Nov 16, 2007·Journal of Clinical Immunology·Nam-Hee KwonDong-Chull Choi
Feb 6, 2010·Journal of Cellular and Molecular Medicine·Rutger J WierdaPeter J van den Elsen
Apr 15, 2011·Immunological Reviews·Roza I NurievaChen Dong
Jan 3, 2013·Immunology·Taku Naito, Ichiro Taniuchi
May 28, 2016·European Journal of Immunology·Pranay DograBen Youngblood
Nov 5, 2014·Frontiers in Cell and Developmental Biology·Riya R KanherkarAntonei B Csoka
Apr 9, 2016·Revista chilena de pediatría·José A Castro-RodríguezPaola Casanello
Jun 6, 2018·The Journal of Biological Chemistry·Sharon A McGrath-MorrowBenjamin D Singer
May 16, 2012·The Journal of Biological Chemistry·Rajan M ThomasAndrew D Wells
Mar 22, 2014·Neuromolecular Medicine·Cem Ismail KüçükaliErdem Tüzün
Jan 17, 2015·Nucleic Acids Research·Bi-Huei YangJochen Huehn
Mar 20, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shin-ichi HashimotoKouji Matsushima
Jan 11, 2012·Antioxidants & Redox Signaling·Nidhi ChaudharySamit Chattopadhyay
Mar 14, 2013·Antioxidants & Redox Signaling·Gene H KimStephen L Archer

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