DNA methylation level of cyp19a1a and Foxl2 gene related to their expression patterns and reproduction traits during ovary development stages of Japanese flounder (Paralichthys olivaceus)

Gene
YuFeng SiZhengju Huang

Abstract

Foxl2 and cyp19a1a genes are crucial for the ovarian development, and Foxl2 could play a direct regulatory role on cyp19a1a transcription. In this study, we aimed to study DNA methylation status and mRNA expression patterns of Foxl2 and cyp19a1a genes during ovarian development of female Japanese flounder. The relative expression level of cyp19a1a and Foxl2 gene during the gonadal development stages was measured by quantitative PCR. Moreover, DNA methylation status in the promoter and coding regions of the two genes was detected by bisulfite sequencing. The estradiol-17β (E2) was measured by radioimmunoassay. The results showed low expression levels of cyp19a1a and Foxl2 genes in stages II and V, while the highest expression levels were detected in stage IV. The variation trend of the methylation level of all CpG sites in promoter and exon 1 of cyp19a1a gene and three CpG rich regions in coding region of Foxl2 gene was negatively associated with their expression levels during the ovarian development. In addition, two CpG sites in promoter and seven CpG sites in exon 1 of cyp19a1a were on the putative transcription factors binding sequence. Further studies showed that the forkhead domain, which is important for Foxl2 binding to ...Continue Reading

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Citations

Feb 3, 2016·Marine Genomics·David C H Metzger, Patricia M Schulte
Jan 26, 2017·Aquatic Toxicology·Tingting GeDejun Huang
Feb 22, 2017·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Zhaofei FanFeng You
Oct 13, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yang YangJun Li
Jan 21, 2020·The Journal of Steroid Biochemistry and Molecular Biology·Congcong ZouFeng You

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