DNA Methylation Markers Improve the Sensitivity of Endoscopic Retrograde Cholangiopancreatography-Based Brushing Cytology in Extrahepatic Cholangiocarcinoma

Technology in Cancer Research & Treatment
Varayu PrachayakulPeti Thuwajit

Abstract

Endoscopic retrograde cholangiopancreatography with brushed cytology is still the standard method for the diagnosis of extrahepatic cholangiocarcinoma in obstructive jaundice; however, the diagnostic yield is limited. To improve the diagnostic sensitivity, DNA methylation analysis is an attractive candidate, since this may constitute a stable marker in brushed specimens. Therefore, this study aims to evaluate the importance of such epigenetic markers in brushed biliary cells from patients with obstructive jaundice for the diagnosis of extrahepatic cholangiocarcinoma. The cells examined were those that were left over from brushed cytology done during routine endoscopic retrograde cholangiopancreatography of patients with extrahepatic cholangiocarcinoma. The methylation states of HOXA1, RASSF1A, P16, and NEUROG1 genes in extrahepatic cholangiocarcinoma were measured by quantitative methylation-specific polymerase chain reaction and compared between brushed biliary cells and normal gall bladder epithelial cells. The results showed that the sensitivity of the methylation index measurements of HOXA1 and NEUROG1 genes from brushed samples was markedly superior to that of standard cytology. In conclusion, measurement of the DNA methyl...Continue Reading

References

Oct 23, 1997·Microscopy Research and Technique·J Gilloteaux
Sep 5, 2002·The Journal of Pathology·Andrea TannapfelChristian Wittekind
Oct 26, 2002·Journal of Hepatology·Nathalie WongPhilip J Johnson
Oct 7, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Bin YangDouglas P Clark
Dec 14, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·S FojaW G Ballhausen
May 13, 2008·Cancer Letters·Jens M TeodoridisRobert Brown
Dec 19, 2008·The Journal of Biological Chemistry·Joseph AvruchFan Xia
Sep 19, 2009·Virchows Archiv : an International Journal of Pathology·Baek-hui KimGyeong Hoon Kang
Feb 18, 2011·The American Journal of Gastroenterology·Andreas HerbstFrank T Kolligs
Feb 1, 2012·Cancer Letters·Edward S GreenbergDave S B Hoon
Mar 7, 2012·Langenbeck's Archives of Surgery·Lena SibuleskyTushar Patel
Dec 24, 2013·Clinics and Research in Hepatology and Gastroenterology·Jifeng XiangJiming Wang
Mar 4, 2014·Lancet·Nataliya Razumilava, Gregory J Gores
Jul 13, 2014·Biochemical and Biophysical Research Communications·Hiromu SuzukiMasahiro Kai
Nov 4, 2015·Nature Reviews. Gastroenterology & Hepatology·Christopher L BowlusM Eric Gershwin
Mar 13, 2016·Blood Reviews·Benjamin E PaluchElizabeth A Griffiths

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Citations

Aug 10, 2020·Diagnostic Cytopathology·Fatima Zahra AlyRobert Klein
Mar 9, 2021·Expert Review of Gastroenterology & Hepatology·Brian M Fung, James H Tabibian
Sep 18, 2020·JHEP Reports : Innovation in Hepatology·Hege Marie VedeldGuro Elisabeth Lind
Jul 16, 2021·Gastrointestinal Endoscopy·Varayu PrachayakulPeti Thuwajit

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