DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma

Cancer Management and Research
Fada XiaXinying Li

Abstract

Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy and its incidence has increased over the last few decades. The molecular mechanisms underlying PTC tumorigenesis and progression are still unclear. The microRNA (miRNA) expression patterns of PTC were revealed by miRNA microarray analysis and validated with The Cancer Genome Atlas (TCGA) data. Promoter DNA methylation rates of miR-204 were analyzed by Agena Methylation MassAR-RAY analysis and validated with TCGA data. The underlying molecular mechanisms of miR-204 involved in PTC were studied by bioinformatics analyses. A total of 181 differentially expressed miRNAs (89 downregulated and 92 upregulated miRNAs) between PTC and normal tissues were detected in this study. We identified miR-204 as one of the most significantly downregulated miRNAs in PTC. Downregulation of miR-204 was related to PTC extrathyroidal extension, high T-stage, lymph metastasis, BRAF V600E mutation, and aggressive tall cell variant. The Agena MassARRAY results indicated that 12 CpG sites located at the promoter of miR-204 were hypermethylated in PTC tissues compared to normal tissues. The promoter methylation rates of miR-204 in PTC were negatively correlated with the expression le...Continue Reading

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Datasets Mentioned

BETA
GSE113629

Methods Mentioned

BETA
PCR
chip
chips
feature extraction
Assay
PCRs
in vitro transcription
RNAseq

Software Mentioned

MethHC
EpiTYPER
DAVID
TreeView
ggplot2
Cluster
GSEA JAVA
Agilent feature extraction
Agena Methylation Mas - sARRAY
R

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