DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation

The Korean Journal of Parasitology
Eun-Kyung MoonHyun-Hee Kong

Abstract

Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated wi...Continue Reading

References

Jul 7, 2000·The Journal of Antimicrobial Chemotherapy·N A TurnerD Lloyd
Mar 28, 2002·Cellular and Molecular Life Sciences : CMLS·J T AttwoodB C Richardson
Aug 3, 2002·Oncogene·Keith D Robertson
Apr 1, 2005·International Journal of Cancer. Journal International Du Cancer·Jun Hee LimTaeg Kyu Kwon
Aug 5, 2008·Eukaryotic Cell·Eun-Kyung MoonHyun-Hee Kong
Jun 30, 2009·Molecular and Biochemical Parasitology·Eun-Kyung MoonHyun-Hee Kong
Oct 1, 2009·Nature Reviews. Genetics·Peter A Jones, Gangning Liang
Oct 15, 2010·Nature Biotechnology·Anna Portela, Manel Esteller
Aug 11, 2011·Nature Communications·Kathrin K GeyerKarl F Hoffmann
Feb 23, 2012·The Korean Journal of Parasitology·Eun-Kyung MoonHyun-Hee Kong
Mar 20, 2012·Molecular and Biochemical Parasitology·Su-Min SongYeonchul Hong
Aug 25, 2012·Molecular and Biochemical Parasitology·Eun-Kyung MoonHyun-Hee Kong
Dec 18, 2013·Cell Host & Microbe·Nadia PontsKarine G Le Roch
May 23, 2014·The Korean Journal of Parasitology·Eun-Kyung MoonHyun-Hee Kong
May 18, 2016·The Korean Journal of Parasitology·Eun-Kyung MoonHyun-Hee Kong

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Citations

Apr 3, 2021·Frontiers in Genetics·Agnes K M Weiner, Laura A Katz
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·H FatimahHazlina A Zakeri

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Methods Mentioned

BETA
PCR

Software Mentioned

Methprimer

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