DNA methylation of miRNA coding sequences putatively associated with childhood obesity

Pediatric Obesity
María Luisa MansegoGENOI members

Abstract

Epigenetic mechanisms may be involved in obesity onset and its consequences. The aim of the present study was to evaluate whether DNA methylation status in microRNA (miRNA) coding regions is associated with childhood obesity. DNA isolated from white blood cells of 24 children (identification sample: 12 obese and 12 non-obese) from the Grupo Navarro de Obesidad Infantil study was hybridized in a 450 K methylation microarray. Several CpGs whose DNA methylation levels were statistically different between obese and non-obese were validated by MassArray® in 95 children (validation sample) from the same study. Microarray analysis identified 16 differentially methylated CpGs between both groups (6 hypermethylated and 10 hypomethylated). DNA methylation levels in miR-1203, miR-412 and miR-216A coding regions significantly correlated with body mass index standard deviation score (BMI-SDS) and explained up to 40% of the variation of BMI-SDS. The network analysis identified 19 well-defined obesity-relevant biological pathways from the KEGG database. MassArray® validation identified three regions located in or near miR-1203, miR-412 and miR-216A coding regions differentially methylated between obese and non-obese children. The current work...Continue Reading

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Citations

Apr 4, 2017·The British Journal of Dermatology·M C Ovejero-BenitoF Abad-Santos
Jul 13, 2018·Brain and Behavior·Omar Ramos-LopezUNKNOWN MENA Project
Mar 21, 2018·Experimental and Therapeutic Medicine·Peijie ChenYongqing Lai
Nov 12, 2019·Journal of Cellular Biochemistry·Rafael Silva LimaPaula Rocha Moreira
Jan 17, 2019·BioMed Research International·Yuxiang HuangYongqiang Li
May 7, 2019·Genes & Nutrition·Omar Ramos-LopezUNKNOWN MENA project
Jun 3, 2021·International Journal of Molecular Sciences·Farha RamzanRichard F Mithen

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