DNA methylation of the homeobox genes PITX2 and SHOX2 predicts outcome in non-small-cell lung cancer patients

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
Dimo DietrichAlex Soltermann

Abstract

Biomarkers that facilitate prediction of disease progression in lung cancer patients might be clinically valuable in optimizing individualized therapy. In this study, the ability of the DNA methylation biomarkers PITX2 and SHOX2 to predict disease outcome in lung cancer patients has been evaluated. Quantitative, methylation-specific (HeavyMethyl), real-time polymerase chain reaction assays were used to measure DNA methylation of PITX2 and SHOX2 in bisulfite-converted DNA from formalin-fixed, paraffin-embedded tissues from 474 non-small-cell lung cancer patients. In univariate Cox Proportional Hazard analysis, high methylation of SHOX2 and PITX2 was a significant predictor of progression-free survival [SHOX2: n=465, hazard ratio (HR)=1.395 (1.130 to 1.721), P=0.002; PITX2: n=445, HR=1.312 (1.059 to 1.625), P=0.013]. Patients with low methylation of either PITX2 and/or SHOX2 (n=319) showed a significantly higher risk of disease progression as compared with patients with higher methylation of both genes [n=126; HR=1.555 (1.210 to 1.999), P=0.001]. This was particularly true for the subgroup of patients receiving no adjuvant radiotherapy or chemotherapy [n=258, HR=1.838 (1.252 to 2.698), P=0.002]. In multivariate analysis, both bio...Continue Reading

References

Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J BlaschkeG A Rappold
Jul 3, 1999·Cancer·F LeviC La Vecchia
Apr 6, 2000·Human Molecular Genetics·M Clement-JonesG A Rappold
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 2, 2003·Annals of the New York Academy of Sciences·Yinhua YuRobert C Bast
May 7, 2003·Trends in Cardiovascular Medicine·Diego Franco, Marina Campione
Sep 2, 2005·Nature Reviews. Genetics·Keith D Robertson
Dec 13, 2005·Nature Clinical Practice. Oncology·Stephen B Baylin
Aug 2, 2006·Journal of Neuroendocrinology·M H QuentienA Enjalbert
Dec 13, 2006·Genes & Development·Angela H TingStephen B Baylin
Feb 27, 2007·Cell·Peter A Jones, Stephen B Baylin
Mar 31, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Dahe LinYanding Zhang
Jul 3, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sabine MaierUNKNOWN European Organisation for Research and Treatment of Cancer (EORTC) PathoBiology group
Oct 9, 2007·Archives of Physiology and Biochemistry·Antonio MarchiniKatja U Schneider
Jan 2, 2008·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Hisao AsamuraUNKNOWN Japanese Joint Committee of Lung Cancer Registry
Mar 5, 2008·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Jun Qian, Pierre P Massion
Aug 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Nadia HarbeckRalf Lesche
Sep 20, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Sandro J MartinsVera L Capelozzi
Dec 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Valsamo K AnagnostouDavid L Rimm
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Oliver HartmannJohn W M Martens
Jan 27, 2009·Developmental Biology·Ramón A Espinoza-LewisYiPing Chen
May 13, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Ana Belén CustodioEduardo Díaz-Rubio
Jun 11, 2009·European Journal of Human Genetics : EJHG·Zeynep Tümer, Daniella Bach-Holm
Jun 24, 2009·PloS One·Pierre P MassionMarileila Varella-Garcia
Oct 10, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jim Jinn-Chyuan SheuJen-Yang Chen
Dec 4, 2009·The New England Journal of Medicine·Rob J van KlaverenHarry J de Koning
Mar 20, 2010·American Journal of Respiratory and Critical Care Medicine·Frank McCaughanPamela H Rabbitts
Mar 23, 2010·The Journal of Molecular Diagnostics : JMD·Philipp SchatzArndt Hartmann
May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jun WangJames F Martin
Jul 9, 2010·CA: a Cancer Journal for Clinicians·Ahmedin JemalElizabeth Ward
Aug 26, 2010·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Hiroyuki SakuraiUNKNOWN Japanese Joint Committee for Lung Cancer Registration
Sep 11, 2010·International Journal of Cancer. Journal International Du Cancer·Li-Ru HeDan Xie
Mar 24, 2011·BMC Cancer·Katja U SchneiderBernd Schmidt
Apr 5, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Theresia WilbertzSven Perner

❮ Previous
Next ❯

Citations

Sep 13, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·K DarwicheL Freitag
Jul 16, 2013·Annals of Surgical Oncology·Tian YangFeng Shen
Feb 8, 2013·Biomarkers in Medicine·Ioanna BalgkouranidouEvi S Lianidou
Nov 28, 2013·Endocrine-related Cancer·Julia HoeferMartin Puhr
Oct 29, 2013·Clinical & Experimental Metastasis·I VelaE M Gardiner
Aug 24, 2013·Expert Review of Respiratory Medicine·Michael FleischhackerBernd Schmidt
Sep 18, 2014·Genes·Thomas Mikeska, Jeffrey M Craig
Nov 8, 2012·International Journal of Cancer. Journal International Du Cancer·Jia-Xing ZhangYuan Zhao
May 28, 2015·Molecular Diagnosis & Therapy·Lele SongYuemin Li
Jun 18, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Imran NawazIngemar Ernberg
Nov 9, 2016·Genomics·Maria Fernanda Setúbal Destro RodriguesFabio Daumas Nunes
Feb 15, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G-H ZhangG-J Yue
Jul 22, 2018·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Ying-Ying XuQiang Zhang
Nov 30, 2016·Clinical Epigenetics·René A M DirksHendrik Marks
Jun 12, 2019·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Ju HuangBo Zhang
Jul 29, 2017·Clinical and Experimental Medicine·Yunbao PanYirong Li
Nov 25, 2019·Cancer Letters·Ayumu AsaiHideshi Ishii
Feb 19, 2021·Clinical Epigenetics·Torsten GoldmannOle Ammerpohl
Apr 13, 2021·Cellular and Molecular Life Sciences : CMLS·Thai Q Tran, Chrissa Kioussi
Jul 6, 2021·Current Genomics·Yoonki Hong, Woo Jin Kim
Oct 1, 2021·Journal of Clinical Laboratory Analysis·Huiqin JiangWei Guo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.