DNA methylation patterns of RAR-β2 and RASSF1A gene promoters in FNAB samples from Greek population with benign or malignant breast lesions.

Diagnostic Cytopathology
Niki KougioumtsidouLeonidas Zepiridis

Abstract

Promoter hypermethylation is common in Breast Cancer (BC) with studies mainly in histological specimens showing frequent methylation of tumor suppressor genes (TSGs) compared with normal tissues. The aim of this study was to estimate the frequency of promoter methylation of RAR-β2 and RASSF1A genes in breast FNAB material aiming to evaluate the methylation status of these two genes as biomarker for detecting BC in Greek population. FNAB material from 104 patients was collected for cytological evaluation and epigenetic analysis. DNA was extracted and subjected to bisulfite conversion. A methylation-specific PCR was carried out and the final products were separated with electrophoresis in 2% agarose gels. From 104 samples, RASSF1A hypermethylation was observed in 78 (75%) and RAR-β2 hypermethylation in 64 (61.6%). 84% and 78% of the cases diagnosed with breast malignancy (n = 50) were methylated for RASSF1A and RAR-β2, respectively. Methylated RASSF1A and RAR-β2 were also detected in 88.3% and 76.5% in samples diagnosed as suspicious for malignancy (n = 17) and in 57.2% of samples diagnosed with atypia (n = 14). The Odds Ratio for breast malignancy was 4.545 in patients with RASSF1A hypermethylation and 9.167 in patients with RAR...Continue Reading

References

Oct 1, 1976·Science·P C Nowell
Nov 11, 1996·Biochimica Et Biophysica Acta·A ToulouseW E Bradley
May 18, 2000·Journal of the National Cancer Institute·M WidschwendterC Marth
May 3, 2001·Journal of the National Cancer Institute·D G BurbeeJ D Minna
Oct 3, 2002·Oncogene·Limin LiuGerd P Pfeifer
Feb 21, 2003·International Journal of Cancer. Journal International Du Cancer·Michael W Y ChanKa Fai To
Apr 4, 2003·International Journal of Cancer. Journal International Du Cancer·Mei Y YuKai F To
Nov 6, 2003·International Journal of Cancer. Journal International Du Cancer·Mary Jo FacklerPedram Argani
Nov 14, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Robert T PuDouglas P Clark
Sep 28, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Essel DulaimiPaul Cairns
Mar 25, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masaru ShinozakiBret Taback
Apr 13, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Gregory R BeanVictoria L Seewaldt
Sep 2, 2005·Molecular and Cellular Biology·L van der WeydenA Bradley
Aug 19, 2007·Biochimica Et Biophysica Acta·Louise van der Weyden, David J Adams
Sep 15, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·David M EuhusCheryl M Lewis
Sep 20, 2007·Journal of Cell Science·Howard DonningerGeoffrey J Clark
Nov 14, 2007·Epigenetics : Official Journal of the DNA Methylation Society·Shruti ShuklaRanju Ralhan
May 17, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·David M EuhusCheryl M Lewis
Jul 30, 2008·Genes, Chromosomes & Cancer·Jane J PappasW E C Bradley
Apr 21, 2009·Clinical Biochemistry·Magdalini KioulafaEvi S Lianidou
Aug 7, 2009·Journal of Cancer Research and Clinical Oncology·Sondes Karray-ChouayekhRaja Mokdad-Gargouri
Nov 26, 2009·Biochimica Et Biophysica Acta·Andriy Marusyk, Kornelia Polyak
Feb 25, 2010·Heredity·V Bollati, A Baccarelli
Jul 16, 2010·Cancer Epidemiology·Jennifer D BrooksAnne Zeleniuch-Jacquotte
Dec 2, 2010·Virchows Archiv : an International Journal of Pathology·So Yeon ParkGyeong Hoon Kang
Jun 29, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Barbara NorquistElizabeth M Swisher
Aug 13, 2011·Breast Cancer Research and Treatment·Yoon Hee ChoRegina M Santella
Nov 30, 2011·Epigenomics·Jorge Alejandro Alegría-TorresValentina Bollati
Jan 5, 2012·Nature Reviews. Genetics·Robert Feil, Mario F Fraga
Feb 3, 2012·Cancer Biomarkers : Section a of Disease Markers·Katarina SebovaIvana Fridrichova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.