DNA Methylation Profiling of Breast Cancer Cell Lines along the Epithelial Mesenchymal Spectrum-Implications for the Choice of Circulating Tumour DNA Methylation Markers

International Journal of Molecular Sciences
Anh Viet-Phuong LeAlexander Dobrovic

Abstract

(1) Background: Epithelial⁻mesenchymal plasticity (EMP) is a dynamic process whereby epithelial carcinoma cells reversibly acquire morphological and invasive characteristics typical of mesenchymal cells. Identifying the methylation differences between epithelial and mesenchymal states may assist in the identification of optimal DNA methylation biomarkers for the blood-based monitoring of cancer. (2) Methods: Methylation-sensitive high-resolution melting (MS-HRM) was used to examine the promoter methylation status of a panel of established and novel markers in a range of breast cancer cell lines spanning the epithelial⁻mesenchymal spectrum. Pyrosequencing was used to validate the MS-HRM results. (3) Results: VIM, DKK3, and CRABP1 were methylated in the majority of epithelial breast cancer cell lines, while methylation of GRHL2, MIR200C, and CDH1 was restricted to mesenchymal cell lines. Some markers that have been used to assess minimal residual disease such as AKR1B1 and APC methylation proved to be specific for epithelial breast cell lines. However, RASSF1A, RARβ, TWIST1, and SFRP2 methylation was seen in both epithelial and mesenchymal cell lines, supporting their suitability for a multimarker panel. (4) Conclusions: Profilin...Continue Reading

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Citations

Jul 8, 2020·Journal of Cellular and Molecular Medicine·Reza KhayamiMohammad Amin Kerachian
Aug 20, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xuefei FengPeijun Liu
Aug 8, 2019·International Journal of Molecular Sciences·Dana DvorskáZuzana Danková
Apr 23, 2021·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Iman AkhlaghipourMeysam Moghbeli

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Datasets Mentioned

BETA
GSE12790
MFM-223

Methods Mentioned

BETA
PCR

Software Mentioned

Qseq

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