DNA methylation signatures of breast cancer in peripheral T-cells

BMC Cancer
Surabhi ParasharShafaat A Rabbani

Abstract

Immune surveillance acts as a defense mechanism in cancer, and its disruption is involved in cancer progression. DNA methylation reflects the phenotypic identity of cells and recent data suggested that DNA methylation profiles of T cells and peripheral blood mononuclear cells (PBMC) are altered in cancer progression. We enrolled 19 females with stage 1 and 2, nine with stage 3 and 4 and 9 age matched healthy women. T cells were isolated from peripheral blood and extracted DNA was subjected to Illumina 450 K DNA methylation array analysis. Raw data was analyzed by BMIQ, ChAMP and ComBat followed by validation of identified genes by pyrosequencing. Analysis of data revealed ~ 10,000 sites that correlated with breast cancer progression and established a list of 89 CG sites that were highly correlated (p < 0.01, r > 0.7, r < - 0.7) with breast cancer progression. The vast majority of these sites were hypomethylated and enriched in genes with functions in the immune system. The study points to the possibility of using DNA methylation signatures as a noninvasive method for early detection of breast cancer and its progression which need to be tested in clinical studies.

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Citations

Jan 12, 2019·Biomedit︠s︡inskai︠a︡ khimii︠a︡·E V KugaevskayaN I Solovyeva
Jul 4, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xue ZengXiaofang Che
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May 10, 2020·Cancer Research·Daniela Matei, Kenneth P Nephew
Jun 5, 2021·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Hongxia ChenChunYan Yang

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Methods Mentioned

BETA
blood drawn
PCR
biopsy

Software Mentioned

ChAMP
Plotter
ComBat
Limma
BMIQ
KM
Ingenuity Pathway Analysis ( IPA )
PyroMark® Q24
GraphPad
IDAT

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