DNA methyltransferase detection based on digestion triggering the combination of poly adenine DNA with gold nanoparticles

Biosensors & Bioelectronics
Pei LiuShiyun Ai

Abstract

DNA methyltransferase (MTase) has received a large amount of attention due to its catalyzation of DNA methylation in both eukaryotes and prokaryotes, which has a close relationship to cancer and bacterial diseases. Herein, a novel electrochemical strategy based on Dpn I digestion triggering the combination of poly adenine (polyA) DNA with a gold nanoparticles functioned glassy carbon electrode (AuNPs/GCE), is developed for the simple and efficient detection of DNA MTase and inhibitor screening. Only one methylene blue (MB)-labeled DNA hairpin probe and two enzymes are involved in this designed method. In the presence of Dam MTase, the hairpin probe can be methylated and then cleaved by the restriction endonuclease. Thus, a MB-labeled polyA signal-stranded DNA product is introduced to the surface of AuNPs/GCE through the effect between polyA and AuNPs, resulting in an obvious electrochemical signal. On the contrary, in the absence of Dam MTase, the DNA probe cannot be cleaved and a relatively small electrochemical response can be observed. As a result, the as-proposed biosensor offered an efficient way for Dam MTase activity monitoring with a low detection of 0.27 U/mL, a wide linear range and good stability. Additionally, this ...Continue Reading

Citations

Oct 25, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Juan WuRenbing Tian
Aug 20, 2016·Lab on a Chip·Ryoji Kurita, Osamu Niwa
Mar 1, 2018·Annual Review of Analytical Chemistry·Dekai YeChunhai Fan
Aug 4, 2018·Analytical and Bioanalytical Chemistry·Susana CampuzanoJosé M Pingarrón
Sep 5, 2019·Current Medicinal Chemistry·Shu ZhangJunsong Zheng

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