DNA microarray analysis of chromosomal susceptibility regions to identify candidate genes for allergic disease: a pilot study

Acta Oto-laryngologica
M BensonLars Olaf Cardell

Abstract

To examine whether DNA microarray analysis of chromosomal susceptibility regions for allergy can help to identify candidate genes. Nasal biopsies were obtained from 23 patients with allergic rhinitis and 12 healthy controls. RNA was extracted from the biopsies and pooled into three patient and three control pools. These were then analysed in duplicate with DNA microarrays containing 12626 genes. Candidate genes were further examined in nasal biopsies (real-time polymerase chain reaction) and blood samples (single nucleotide polymorphisms) from other patients with allergic rhinitis and from controls. A total of 37 differentially expressed genes were identified according to criteria involving both the size and consistency of the gene expression levels. The chromosomal location of these genes was compared with the chromosomal susceptibility regions for allergic disease. Using a statistical method, five genes were identified in these regions, including serine protease inhibitor, Kazal type, 5 (SPINK5) and HLA-DRB2. The relevance of these genes was examined in other patients with allergic rhinitis and in controls; none of the genes were differentially expressed in nasal biopsies. Moreover, no association between allergic rhinitis an...Continue Reading

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Citations

Aug 4, 2006·Forensic Science International : Synergy·Bin JiangYumin Mao
Apr 12, 2007·American Journal of Rhinology·Alexander D KaratzanisGeorge A Velegrakis
Nov 28, 2007·Molecular and Cellular Biology·Lance LeeMark D Fleming

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