PMID: 1206799Jan 1, 1975Paper

DNA-minus temperature-sensitive mutants of adenovirus type 5 help adenovirus-associated virus replication

Journal of Virology
S E StrausJ A Rose

Abstract

Efficient potentiation of adenovirus-associated viruses (AAV) replication occurs in coinfections with either of two DNA-minus temperature-sensitive mutants of adenovirus type 5 (Ad5), ts125 and ts149. The helper activity of these mutants does not result from leakiness. At the nonpermissive termperature (39.5 C) there was little or no detectable adenovirus DNA synthesis, and only a relatively low level of adenovirus transcription was observed. However, the synthesis of AAV DNA and RNA and the yield of infectious AAV were comparable in amounts to those found when wild-type Ad5 was the helper. Furthermore, an apparent lag in the initiation of AAV transcription after the onset of AAV DNA synthesis was seen in coinfections with both wild type or ts125. These findings strongly suggest that the adenovirus factor(s) required for AAV multiplication is produced early in the adenovirus DNA replication, this requirement does not include all factors directly needed for adenovirus DNA synthesis.

References

Jun 14, 1967·Journal of Molecular Biology·B Hirt
Sep 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·N R BlacklowM S McClanahan
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J Wolfson, D Dressler
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·F J KoczotJ A Rose
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C F GaronJ A Rose
Mar 19, 1973·Biochimica Et Biophysica Acta·H D Mayor, J Ratner
Dec 1, 1972·The Journal of General Virology·W C RusselJ F Williams
Sep 1, 1971·Virology·U Lundholm, W Doerfler
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M D HogganW P Rowe
Aug 23, 1966·Biochemical and Biophysical Research Communications·S O Warmaar, J A Cohen
Oct 1, 1968·Journal of Virology·J A RoseA J Shatkin

❮ Previous
Next ❯

Citations

Feb 18, 2004·Human Gene Therapy·Nicola J PhilpottErik Falck-Pedersen
Mar 16, 2007·Journal of Virology·Kevin NashNicholas Muzyczka
Nov 3, 2014·Annual Review of Virology·R Jude Samulski, Nicholas Muzyczka
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K I Berns, R M Linden
Feb 1, 1994·Journal of Virology·T H NiN Muzyczka
Nov 1, 1986·Journal of Virology·C A LaughlinH C Coon
Nov 1, 1982·Journal of Virology·R A McPhersonJ A Rose
Jul 1, 1984·Journal of Virology·H E TakiffS E Straus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.