DNA mismatch repair enzyme hMSH2 in malignant melanoma: increased immunoreactivity as compared to acquired melanocytic nevi and strong mRNA expression in melanoma cell lines

The Histochemical Journal
K RassJ Reichrath

Abstract

Mutations in the mismatch DNA repair gene human MutS homologen 2 (hMSH2) are causative for microsatellite instability and carcinogenesis in various human tumours, including hereditary nonpolyposis colorectal cancer. Because microsatellite instability has been detected in malignant melanoma, we have investigated hMSH2 in melanocytic tumours. We found strong nuclear immunoreactivity for hMSH2 that was elevated in malignant melanoma and melanoma metastases as compared to acquired nevi. These findings suggest that increased genomic instability in malignant melanoma is associated with elevated protein levels of this DNA repair enzyme. hMSH2 is not exclusively regulated by proliferative activity in melanocytes, because there was no correlation between staining patterns of hMSH2 and the proliferation marker Ki-67. In contrast, immunoreactivity scores for hMSH2 and p53 were both upregulated in malignant melanocytic tumours. These findings support the concept that hMSH2 gene expression may be regulated in melanocytes by the p53 protein, as has been reported previously in other tissues. Using the reverse transcription-polymerase chain reaction, we detected strong hMSH2 mRNA expression in each of 8 melanoma cell lines analysed (highest am...Continue Reading

Citations

Jun 6, 2003·Oncogene·María S Soengas, Scott W Lowe
Nov 1, 2007·The British Journal of Dermatology·L C YoungV A Tron
Jun 14, 2014·American Journal of Clinical Pathology·Ester AlvinoStefania D'Atri
Sep 28, 2013·PloS One·Irene Rodríguez-HernándezRogelio González-Sarmiento
Aug 23, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maximilian BurgerRobert Stoehr
Jun 29, 2005·Molekuliarnaia biologiia·V I TarasenkoIu M Konstantinov

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