DNA phosphorothioate modification facilitates the dissemination of mcr-1 and blaNDM-1 in drinking water supply systems

Environmental Pollution
Hira KhanXiaohui Bai


The mechanism driving the dissemination of antibiotic resistance genes (ARGs) in drinking water supply systems (DWSSs) with multiple barriers remains poorly understood despite several recent efforts. Phosphorothioate (PT) modifications, governed by dndABCDE genes, occur naturally in various bacteria and involve the incorporation of sulfur into the DNA backbone. PT is regarded as a mild antioxidant in vivo and is known to provide protection against bacterial genomes. We combined quantitative polymerase chain reaction, metagenomic, and network analyses for the water treatment process and laboratory-scale experiments for chlorine treatment using model strains to determine if DNA PT modification occurred in DWSS and facilitated the dissemination of mobilized colistin resistance-1 (mcr-1) and New Delhi metallo-β-lactamase-1 (blaNDM-1) in DWSS. Our results indicated that the relative abundance of dndB increased in the effluent, compared with the influent, in the water treatment plants. Presence of dndB copies had a positive correlation with the concentration of chloramine disinfectant. Network analysis revealed Bdellovibrio as a potential host for MCR genes, NDM genes, and dndB in the DWSS. E. coli DH10B (Wild-type with the dndABCDE ...Continue Reading


Jan 1, 1985·Annual Review of Biochemistry·F Eckstein
Oct 1, 1988·Applied and Environmental Microbiology·M W LeChevallierR G Lee
Oct 6, 2000·Annual Review of Microbiology·U SzewzykK H Schleifer
Dec 3, 2004·Applied and Environmental Microbiology·Xiufen ZhouZixin Deng
Aug 17, 2005·Molecular Microbiology·Xiufen ZhouZixin Deng
Jan 1, 1990·Applied and Environmental Microbiology·A J Schoeffield, H N Williams
Apr 1, 1992·Applied and Environmental Microbiology·J Kelley, H N Williams
Dec 22, 2006·Environmental Science & Technology·Amy PrudenKenneth H Carlson
May 3, 2007·Environmental Microbiology·María Teresa GarcíaYousef Abu Kwaik
Oct 16, 2007·Nature Chemical Biology·Lianrong WangPeter C Dedon
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Apr 15, 2009·Microbial Ecology·Michael TaylorRichard Bentham
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Jul 8, 2009·Applied and Environmental Microbiology·Chuanwu XiJerome Nriagu
Mar 10, 2010·BMC Bioinformatics·Doug HyattLoren J Hauser
Jun 1, 2012·BMC Bioinformatics·Ye DengJizhong Zhou
Jul 10, 2012·Nucleic Acids Research·Xinqiang XieZhijun Wang
Oct 13, 2012·Bioinformatics·Limin FuWeizhong Li
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yong-Guan ZhuJames M Tiedje
Jan 1, 1995·Microbial Ecology·H N WilliamsW A Falkler
Jul 25, 2014·Environmental Science & Technology·Feng-Hua WangYong-Guan Zhu
Jul 8, 2015·The Science of the Total Environment·Xiaohui BaiXiang Xiao
Jul 21, 2015·Scientific Reports·Wei HeLianrong Wang
Nov 26, 2015·The Lancet Infectious Diseases·David L Paterson, Patrick N A Harris
Dec 10, 2015·Applied Microbiology and Biotechnology·Célia M ManaiaOlga C Nunes
Jan 18, 2016·The Lancet Infectious Diseases·Marisa HaenniJean-Yves Madec
Feb 6, 2016·Environmental Science & Technology·Hexing WangQingwu Jiang
Apr 14, 2016·Nature Communications·Peter MenzelAnders Krogh
May 22, 2016·Infectious Disease Clinics of North America·Keith S KayeJian Li
May 28, 2016·Antimicrobial Agents and Chemotherapy·Patrick McGannKurt E Schaecher
May 29, 2016·Applied and Environmental Microbiology·Dinesh M FernandoAyush Kumar
Jul 20, 2016·Antimicrobial Agents and Chemotherapy·Jose F Delgado-BlasBruno Gonzalez-Zorn
Aug 11, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Beiwen ZhengLanjuan Li
Feb 22, 2017·Scientific Reports·Tingting WuYi-Lei Zhao
Jun 13, 2017·Nature Chemical Biology·Stefanie KellnerPeter C Dedon
Jun 20, 2017·Environmental Science & Technology·Qing-Lin ChenLi Cui
Jun 29, 2017·Annual Review of Microbiology·Matthew D Surette, Gerard D Wright
Mar 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tong TongShi Chen
Dec 13, 2018·Journal of Water and Health·Qing WuXinhua Zhao

Related Concepts

Water Consumption
Escherichia coli
Gene Clusters
Laboratory Procedures
Polycyclic Hydrocarbons, Aromatic

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.