PMID: 2509660Oct 1, 1989Paper

DNA photoreactivating enzyme from human tissues

Journal of Photochemistry and Photobiology. B, Biology
S E OgutB M Sutherland

Abstract

Photoreactivating enzyme activity has been quantitated in human fetal skin, kidney, lung, liver, brain and intestine, and in neonatal human foreskin. In all the tissues examined there were at least two activities: one nominally greater than 10,000 Da, and one nominally less than 10,000 Da. Both can photolyze pyrimidine dimers in DNA using only light of wavelengths greater than 320 nm, thus excluding tryptophan-mediated dimer splitting as an important mechanism for these activities. The activities are inactivated by digestion with trypsin or pronase, and decreased partially or totally by heating to 65 degrees C. The activities from all six tissues, as well as that from neonatal foreskin, act catalytically in dimer photolysis. The properties of macromolecular size, heat lability, protease sensitivity and catalytic pyrimidine dimer photolysis by a non-tryptophan-mediated mechanism correspond to those of a true photoreactivating enzyme.

References

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Citations

Sep 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·F R de Gruijl, L Roza
Apr 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·F ZölzerK A Samoilova
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y F LiA Sancar
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·B M Sutherland, P V Bennett
Aug 1, 1993·Experimental Dermatology·A TanewH Hönigsmann
Oct 27, 1997·Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography·N M AmmashG K Danielson
Sep 5, 2002·Photodermatology, Photoimmunology & Photomedicine·Betsy M SutherlandR W Gange
Mar 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·P F HeelisA Sancar
Jul 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·D H Hug, J K Hunter

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