DNA ploidy measurement in oral leukoplakia: different results between flow and image cytometry

Oral Oncology
E R E A BrounsI van der Waal

Abstract

The estimated prevalence of oral leukoplakia is worldwide approximately 2%, with an annual malignant transformation rate of approximately 1%. The aim of the present study was to evaluate the possible contribution of ploidy measurement to the prediction of the clinical course, in a well defined cohort of patients with oral leukoplakia. Ploidy was measured by both flow cytometry (FCM-DNA) and image cytometry (ICM-DNA) and we focussed on the comparison of the two different techniques to determine ploidy. A total of 41 patients have been included, with a mean age of 59 years (range 36-78 years). With FCM-DNA, three lesions were aneuploid, with ICM-DNA, 19 lesions were aneuploid. DNA ploidy was compared with clinicopathological and patients parameters. There were no statistically significant differences between DNA ploidy and any patient factor with both FCM-DNA and ICM-DNA. Using FCM-DNA, DNA aneuploid lesions showed statistically significant more dysplasia (p=0.04) than diploid lesions. Furthermore, DNA aneuploid lesions were more frequently encountered at high-risk locations (p=0.03) as being determined with FCM-DNA. These relations were not found when DNA ploidy was determined with ICM-DNA.

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Citations

Apr 17, 2016·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Marcelo SperandioEdward W Odell
May 15, 2018·Oral Diseases·Janaina S GhizoniEdward W Odell
Nov 21, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Letícia Martins GuimarãesCarolina Cavalieri Gomes
Nov 6, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Hans Prakash SathasivamMax Robinson
Jan 1, 2015·The Journal of Contemporary Dental Practice·Sajith VellappallySukumaran Anil
May 2, 2018·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Nikolaos G NikitakisJohn J Sauk

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