PMID: 3760241Sep 1, 1986Paper

DNA ploidy patterns and cytokinetics of non-Hodgkin's lymphoma

Journal of Clinical Pathology
S K JunejaJ D Parkin

Abstract

Flow cytometry studies for cellular DNA analysis were performed in 115 cases of non-Hodgkin's lymphoma, 53 of which had not received any prior chemotherapy or radiotherapy. DNA content was measured in ethanol fixed cells stained with chromomycin A3. According to the criteria of the International Working Formulation there were 43 low grade, 58 intermediate grade, and eight high grade lymphomas; six cases were in the miscellaneous group. Seventy seven (67%) had only diploid DNA content. Thirty eight (33%) showed DNA aneuploidy; 20 of these had been previously treated with chemotherapy or radiotherapy, or both. DNA aneuploidy was seen as hyperdiploidy in all cases except one, and it varied from slightly hyperdiploid to tetraploid. The incidence of aneuploidy increased significantly with increasing histological grade (p = 0.0002) and was not related to previous treatment. The low, intermediate, and high grade lymphomas had 14% (six of 43), 47% (27 of 58), and 62.5% (five of eight) cases, respectively, that showed DNA aneuploidy. The percentage of cells in S phase increased significantly with a higher histological grade (p less than 0.0001). The median S fraction in the low, intermediate, and high grade lymphomas was 1.0 (0.5 to 10)...Continue Reading

References

Nov 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W Taylor, B K Milthorpe
Apr 1, 1984·The Journal of Clinical Investigation·S E ShackneyJ W Parker

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Citations

Jan 1, 1991·Journal of Clinical Laboratory Analysis·D C Aziz, J B Peter
Mar 1, 1990·Journal of Clinical Pathology·P A Hall, D A Levison
May 1, 1994·[Rinshō ketsueki] The Japanese journal of clinical hematology·K Nanba
Feb 1, 1989·British Journal of Haematology·J S WileyG P Jamieson
Mar 4, 2005·Journal of Materials Science. Materials in Medicine·Hae-Won KimHyoun-Ee Kim
Oct 3, 2006·The Journal of Oral Implantology·Zeev Ormianer, Guido Schiroli
Nov 1, 1990·Hematological Oncology·R AineM Alavaikko

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