PMID: 9663666Jul 15, 1998Paper

DNA ploidy, proliferative activities, and immunophenotype of malignant lymphoma: application of flow cytometry

Head & Neck
A HoriiT Kubo

Abstract

To explore the flow cytometric diagnosis of malignant lymphoma, we examined the deoxyribonucleic acid (DNA) ploidy, proliferative activities, and immunophenotype of surgical biopsy- and fine-needle aspiration (FNA)-derived materials. Our goal was to determine the possibility of making a diagnosis of malignant lymphoma by flow cytometric analysis of FNA-derived materials. The DNA ploidy and proliferative indices including the percentage of S-phase fraction (SPF), G2 + M fraction (G2M), and Ki-67-positive fraction (Ki-67) were analyzed on the fresh materials from 84 consecutive patients with suspected malignant lymphoma. Flow cytometric analysis of surface antigens was simultaneously performed. Fourteen of the patients underwent FNA and subsequent surgical biopsy of the same lymph nodes for flow cytometric analysis. The proliferative indices of intermediate-grade non-Hodgkin's lymphomas (NHL) (n = 28) and high-grade NHL (n = 23) were significantly higher than those of the reactive hyperplasia (n = 25). The total for SPF + G2M of 6% was a satisfactory threshold for differentiating these NHL from reactive hyperplasia (sensitivity of 84%, specificity of 88%, and accuracy of 86%). However, low-grade NHL (n = 3) and Hodgkin's lymphoma...Continue Reading

References

Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·H JoensuuP J Klemi
Oct 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·H JoensuuS Jalkanen
Apr 1, 1993·American Journal of Clinical Pathology·R C Braylan

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Citations

Jun 15, 2010·Cancer Cytopathology·Abdullah E AliGilda da Cunha Santos
Jul 8, 2000·American Journal of Clinical Pathology·T L NicolM J Borowitz
Oct 29, 2003·Cytometry. Part B, Clinical Cytometry·Antoni MartínezNeus Villamor

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