PMID: 2499883Jun 1, 1989Paper

DNA polymerase III gene of Bacillus subtilis

Proceedings of the National Academy of Sciences of the United States of America
B Sanjanwala, A T Ganesan

Abstract

The Bacillus subtilis dnaF (polC) gene that codes for the alpha subunit of the DNA polymerase III holoenzyme has been sequenced. It consists of 4005 base pairs coding for 1335 amino acids (from the start to the stop codon), giving a molecular weight of 151,273. A mutation (azp-12) that confers resistance to the antimicrobial drug 6-(p-hydroxyphenylazo)-uracil is due to a single base change at nucleotide 3523, from TCA to GCA, resulting in a change of the 1175th amino acid, serine, to alanine. It is in the active site and located at the C-terminal part of the enzyme. The amino acid composition in an N-terminal domain has 26% homology to the epsilon subunit coded by the dnaQ gene of Escherichia coli, which is a 3'----5' proofreading exonuclease, supporting an earlier observation that this function is an integral part of the polymerase molecule in B. subtilis.

References

Jan 1, 1977·Annual Review of Biochemistry·N R Cozzarelli
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H Hori, S Osawa
Mar 30, 1976·Molecular & General Genetics : MGG·E LoveN C Brown
Sep 15, 1976·Journal of Molecular Biology·J J Andersen
Jun 17, 1988·Cell·T A Kunkel
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H Maki, A Kornberg
May 1, 1987·Molecular & General Genetics : MGG·R W OttA T Ganesan
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S M CotterillI R Lehman
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H MakiM Sekiguchi
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H EcholsP M Burgers

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Citations

Jan 1, 1993·Molecular & General Genetics : MGG·B Sanjanwala, A T Ganesan
Jan 1, 1990·Pharmacology & Therapeutics·G E Wright, N C Brown
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A MorrisonA Sugino
May 25, 1993·Nucleic Acids Research·E V Koonin, M P Deutscher
Jan 1, 1996·Microbial & Comparative Genomics·S G AnderssonC G Kurland
May 1, 1991·Molecular & General Genetics : MGG·B Sanjanwala, A T Ganesan

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