PMID: 12595253Feb 22, 2003Paper

DNA polymerase X of African swine fever virus: insertion fidelity on gapped DNA substrates and AP lyase activity support a role in base excision repair of viral DNA

Journal of Molecular Biology
Ramón García-EscuderoJosé Salas

Abstract

DNA polymerase X (pol X) from African swine fever virus (ASFV) is the smallest naturally ocurring DNA-directed DNA polymerase (174 amino acid residues) described so far. Previous biochemical analysis has shown that ASFV pol X is a highly distributive, monomeric enzyme, lacking a proofreading 3'-5' exonuclease. Also, ASFV pol X binds intermediates of the single-nucleotide base excision repair (BER) process, and is able to efficiently repair single-nucleotide gapped DNA. In this work, we perform an extensive kinetic analysis of single correct and incorrect nucleotide insertions by ASFV pol X using different DNA substrates: (i) a primer/template DNA; (ii) a 1nt gapped DNA; (iii) a 5'-phosphorylated 1nt gapped DNA. The results obtained indicate that ASFV pol X exhibits a general preference for insertion of purine deoxynucleotides, especially dGTP opposite template C. Moreover, ASFV pol X shows higher catalytic efficiencies when filling in gapped substrates, which are increased when a phosphate group is present at the 5'-margin of the gap. Interestingly, ASFV pol X misinserts nucleotides with frequencies from 10(-4) to 10(-5), and the insertion fidelity varies depending on the substrate, being more faithful on a phosphorylated 1nt g...Continue Reading

References

Nov 25, 1988·Science·B D PrestonL A Loeb
Dec 1, 1987·Virology·A EstevesJ V Costa
Nov 25, 1993·Nucleic Acids Research·R PrasadS H Wilson
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M F GoodmanJ Petruska
Jul 26, 1996·The Journal of Biological Chemistry·C E PiersenR S Lloyd
Aug 8, 1997·The Journal of Biological Chemistry·K B Beckman, B N Ames
Nov 5, 1997·The Journal of Biological Chemistry·A M ChagovetzB D Preston
Jan 10, 1998·The Journal of Biological Chemistry·M OliverosL Blanco
Jun 17, 1998·The Journal of Biological Chemistry·R PrasadS H Wilson
Aug 8, 1998·The Journal of Biological Chemistry·D K SrivastavaS H Wilson
Feb 27, 1999·Current Opinion in Structural Biology·S S ParikhJ A Tainer
Dec 3, 1999·Science·T Lindahl, R D Wood
Mar 14, 2000·Mutation Research·C E PiersenR S Lloyd
Jun 29, 2000·Annual Review of Biochemistry·A K McCulloughR S Lloyd
Aug 10, 2000·Immunological Reviews·C L BenedictJ F Kearney
Aug 18, 2000·Mutation Research·W A Beard, S H Wilson
Aug 31, 2000·Annual Review of Biochemistry·T A Kunkel, K Bebenek
Jul 18, 2001·Journal of the American Chemical Society·A K Showalter, M D Tsai
Aug 30, 2001·The Journal of Biological Chemistry·A A JohnsonK A Johnson
Oct 2, 2001·The Journal of Biological Chemistry·P M BurgersR Woodgate
Oct 27, 2001·Molecular Aspects of Medicine·H J Forman, M Torres
Oct 31, 2001·Nature Structural Biology·M W MaciejewskiG P Mullen
Oct 31, 2001·Nature Structural Biology·A K ShowalterM D Tsai
Feb 1, 2002·The Journal of Biological Chemistry·Miguel García-DíazLuis Blanco

❮ Previous
Next ❯

Citations

Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Benito BañosMiguel de Vega
Mar 13, 2014·Journal of the American Chemical Society·Wen-Jin WuMing-Daw Tsai
Nov 13, 2012·Virus Research·Linda K DixonChris Upton
Nov 5, 2013·Virus Research·Modesto Redrejo-Rodríguez, María L Salas
Oct 29, 2008·Journal of Molecular Biology·Benedetta A Sampoli BenítezTamar Schlick
Dec 10, 2013·Biophysical Journal·Benedetta Sampoli BenítezTamar Schlick
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiwon ChoiSoon B Hwang
Oct 11, 2005·Biophysical Journal·Benedetta A Sampoli BenítezTamar Schlick

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.