PMID: 8615454Apr 1, 1996Paper

DNA probes for the Anopheles punctulatus complex

The American Journal of Tropical Medicine and Hygiene
Nigel W BeebeA Saul

Abstract

Genomic DNA probes were made for two recently identified members of the Anopheles punctulatus complex; Anopheles sp. near punctulatus from Papua New Guinea and Anopheles farauti No. 7 from the Solomon Islands. The probes are species-specific and with the use of 32P labeling sensitive enough so that a squash blot of only a small segment of the mosquito is required for identification. The 119-basepair (bp) probe for An. sp. near punctulatus and the 1,106-bp probe for An. farauti No. 7 have been sequenced in full and the probes have been tested on field collected specimens. These probes now make it possible to distinguish An. sp. near punctulatus and An. farauti No. 7 from the other eight members of the An. punctulatus complex. A pan-species probe was also made from the 18S ribosomal DNA that binds to DNA from all members of the complex. These three probes complete the set required for distinguishing all known members of the An. punctulatus complex by DNA hybridization.

Citations

Feb 6, 2013·Malaria Journal·Thomas R BurkotNeil F Lobo
Sep 5, 2002·Annual Review of Entomology·Jaroslaw Krzywinski, Nora J Besansky
Jan 4, 2007·Medical and Veterinary Entomology·A CywinskaP D N Hebert
Oct 24, 2014·Annual Review of Entomology·Nigel W BeebeRobert D Cooper
Feb 17, 2000·International Journal for Parasitology·N W Beebe, R D Cooper

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