Abstract
The advent of molecular tension probes for real-time mapping of piconewton forces in living systems has had a major impact on mechanobiology. For example, DNA-based tension probes have revealed roles for mechanics in platelet, B cell, T cell, and fibroblast function. Nonetheless, imaging short-lived forces transmitted by low-abundance receptors remains a challenge. This is a particular problem for mechanoimmunology where ligand-receptor bindings are short lived, and a few antigens are sufficient for cell triggering. Herein, we present a mechanoselection strategy that uses locking oligonucleotides to preferentially and irreversibly bind DNA probes that are mechanically strained over probes at rest. Thus, infrequent and short-lived mechanical events are tagged. This strategy allows for integration and storage of mechanical information into a map of molecular tension history. Upon addition of unlocking oligonucleotides that drive toehold-mediated strand displacement, the probes reset to the real-time state, thereby erasing stored mechanical information. As a proof of concept, we applied this strategy to study OT-1 T cells, revealing that the T cell receptor (TCR) mechanically samples antigens carrying single amino acid mutations. ...Continue Reading
References
Apr 13, 2000·Immunology and Cell Biology·S R ClarkeW R Heath
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael T WoodsideSteven M Block
May 22, 2007·Cell·Tasha N SimsMichael L Dustin
Feb 16, 2008·Immunological Reviews·Michael L Dustin
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Georges A AzarPhilippe Bousso
Apr 2, 2010·Nature·Jun HuangCheng Zhu
Jul 2, 2011·Accounts of Chemical Research·Yandong Yin, Xin Sheng Zhao
Nov 1, 2011·Nature Methods·Daniel R StableyKhalid S Salaita
May 25, 2013·Science·Xuefeng Wang, Taekjip Ha
Apr 15, 2014·Cell·Baoyu LiuCheng Zhu
Jun 24, 2014·Current Opinion in Chemical Biology·Manuel F JuetteScott C Blanchard
Jun 27, 2014·PLoS Pathogens·Cristina Godinho-SilvaJ Pedro Simas
Sep 23, 2014·The Journal of Allergy and Clinical Immunology·Inbar Azoulay-AlfaguterAdam Mor
Oct 13, 2014·Nature Methods·Brandon L BlakelyChristopher S Chen
Oct 25, 2014·Nature Communications·Yun ZhangKhalid Salaita
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Dibyendu Kumar DasMatthew J Lang
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yang LiuKhalid Salaita
Jul 22, 2016·ELife·Catherine A HartzellRichard S Lewis
Oct 13, 2016·Cell Reports·Susanne G OberleDietmar Zehn
Dec 8, 2016·The Journal of Cell Biology·Katelyn M Spillane, Pavel Tolar
Mar 9, 2017·The Journal of Biological Chemistry·Kaitao LiCheng Zhu
Mar 11, 2017·Science·Enfu HuiRonald D Vale
May 13, 2017·Science·En CaiMatthew F Krummel
Oct 11, 2017·Nature Reviews. Immunology·Arlene H Sharpe, Kristen E Pauken
Nov 22, 2017·Accounts of Chemical Research·Yang LiuKhalid Salaita
Dec 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yun ZhangKhalid Salaita
Jan 11, 2018·Cell Reports·Viveka MayyaMichael L Dustin
May 19, 2018·Frontiers in Immunology·Brandon L Walling, Minsoo Kim
Jul 28, 2018·Cell·Leah V SibenerK Christopher Garcia
Nov 14, 2018·Nature Immunology·Jinsung HongCheng Zhu
May 10, 2019·Small·Victor Pui-Yan Ma, Khalid Salaita
Citations
Jan 25, 2020·The Journal of Cell Biology·Daniel Blumenthal, Janis K Burkhardt
Sep 14, 2019·Science·Aaron T Blanchard, Khalid Salaita
Oct 20, 2019·Nature Communications·Roxanne GlazierKhalid Salaita
Oct 19, 2019·Nature Methods·Vivien Marx
Jun 9, 2020·Frontiers in Immunology·Nayan D Bhattacharyya, Carl G Feng
Jun 6, 2020·Journal of Cell Science·Francesco BaschieriGuillaume Montagnac
Apr 11, 2020·Biology of the Cell·Mélanie ChabaudClaire Hivroz
Feb 9, 2020·Cells·Muaz RushdiCheng Zhu
Nov 6, 2020·Nucleic Acids Research·Wei-Hung JungYun Chen
Dec 8, 2020·Frontiers in Bioengineering and Biotechnology·Badri Narayanan NarasimhanJenny Malmström
Dec 22, 2020·Advanced Science·Rui LiWeiqiang Chen
Feb 27, 2021·Biosensors & Bioelectronics·Xinxin HangLingqian Chang
Dec 15, 2020·Physical Biology·Aaron T Blanchard, Khalid Salaita
Mar 17, 2021·Trends in Biotechnology·Andreas SchoenitKerstin Göpfrich
Jun 2, 2021·Nature Cell Biology·Hongyun LiZheng Liu
May 13, 2021·Angewandte Chemie·Yuesong HuKhalid Salaita
Jul 10, 2021·Angewandte Chemie·Yuxin DuanKhalid Salaita
Aug 5, 2021·Nature Communications·Aaron BlanchardKhalid Salaita
Nov 5, 2020·Journal of Chemical Theory and Computation·Megan C EngelJonathan P K Doye
Dec 2, 2020·Accounts of Chemical Research·Kewen LeiLi Tang
Aug 20, 2021·Science Advances·Adam B Yasunaga, Isaac T S Li
Jun 13, 2020·ACS Chemical Biology·Ashley K Nguyen, Kristopher A Kilian
Jul 25, 2020·The Journal of Physical Chemistry. B·Paul D CunninghamJoseph S Melinger
Jun 13, 2020·Nano Letters·Chenyi AnWei Chen
Jan 9, 2021·ACS Applied Materials & Interfaces·Roxanne GlazierKhalid Salaita
Sep 9, 2021·Biomaterials Science·Qunye HeZhenbao Liu
Sep 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kevin Y ChenSimon J Davis
Jul 3, 2021·Annual Review of Cell and Developmental Biology·Baoyu LiuBrian D Evavold
Dec 8, 2021·Journal of the American Heart Association·Parvin ForghaniChunhui Xu