DNA-probing for genes coding for denitrification, N2-fixation and nitrification in bacteria isolated from different soils

Zeitschrift Für Naturforschung. C, a Journal of Biosciences
K KloosH Bothe

Abstract

Bacteria isolated from different layers of four soils of the Cologne area were analyzed for denitrifying, nitrifying and N2-fixing isolates by colony hybridization using gene probes. In the soils tested, the percentage of denitrifying bacteria among the total population isolated was 3-8% (in one case exceptionally 15%) and thus small. Denitrifying bacteria were particularly enriched in the upper layer (depth approximately 5 cm) and were present only in low amounts at 25 cm depth in two gleysol soils. Nitrate apparently did not determine the distribution of denitrifying bacteria in these soils. The potential denitrification activity of different soil layers coincided with the distribution pattern of isolates assessed by DNA-probing. The total number of bacteria and of denitrifying isolates was considerably higher in or at the roots of plants than in the bulk, root-free soil adjacent to the plants. The percentage of the isolated aerobic N2-fixing bacteria varied between 0-3%, and these bacteria could be isolated mainly from the upper 5 cm layer. A small portion of the isolates hybridized with the probe coding for part of one subunit of ammonia monooxygenase from Nitrosomonas europaea. The investigation showed that DNA-probing can...Continue Reading

Citations

Sep 7, 2001·Annual Review of Microbiology·G A Kowalchuk, J R Stephen
Nov 26, 2008·Water Environment Research : a Research Publication of the Water Environment Federation·William C Hiatt, C P Leslie Grady
Nov 15, 2000·FEMS Microbiology Reviews·H BotheK Witzel
Jul 31, 2002·Applied and Environmental Microbiology·Christopher RöschHermann Bothe
Jul 18, 2006·FEMS Microbiology Letters·Yuguang ZhangXueduan Liu
Oct 3, 2002·Biochimica Et Biophysica Acta·Laurent Philippot

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