DNA reassociation kinetics in diploid and phylogenetically tetraploid cyprinidae

The Journal of Experimental Zoology
M L Mauro, G Micheli

Abstract

Four diploid and three phylogenetically tetraploid Cyprinidae (Ostariophysi) have been characterized as for nuclear DNA content, modal chromosome number and DNA reassociation kinetics (hydroxyapatite chromatography). Among the diploid species nuclear DNA content (10(-12) g DNA/2C) was 1.62 for Tinca tinca, 1.87 for Scardinius erythrophthalmus, 2.53 for Leuciscus cephalus and 2.75 for Alburnus alburnus, while the phylogenetically tetraploid species Carassius auratus, Barbus barbus and Cyprinus carpio attained 3.40, 3.66 and 3.80 respectively. Modal chromosome number was 2n = 48-50 for diploid individuals and 2n = 100-104 for phylogenetically tetraploid ones. In all the species 5--8% of the genome is represented by highly repetitive and foldback DNA. In DNA reassociation kinetics of phylogenetically tetraploid Cyprinidae a distinct plateau separates an intermediate reassociating sequence fraction (about 22% of the genome; with average repetition frequencies between 1,000 and 1,400) from a slow reassociating one (unique DNA; about 72% of the genome). These two genome fractions are not clearly distinguishable from each other in Cot curves of the diploid Cyprinidae, where a similar plateau is not evident. Since simple ploidy changes...Continue Reading

References

Jan 20, 1977·Nature·S D Ferris, G S Whitt
Apr 3, 1975·Nature·J SchmidtkeW Engel
Oct 1, 1974·Biochemical Genetics·R B FlavellD B Smith
Aug 2, 1972·Nature: New Biology·M Buongiorno-NardelliP A Lava-Sanchez
Jun 15, 1973·Journal of Molecular Biology·E H DavidsonR J Britten
Feb 11, 1972·Science·T Uyeno, G R Smith
Jan 1, 1972·Chromosoma·K Bachmann
May 1, 1971·The Journal of Experimental Zoology·R A Pedersen
Jan 1, 1968·Hereditas·S OhnoN B Atkin
Jan 1, 1968·Chromosoma·J I MuramotoN B Atkin
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER

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Citations

Jan 7, 2009·BMC Evolutionary Biology·Roderick Nigel FinnFrank Nilsen
May 19, 2006·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jing LuoAxel Meyer

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