DNA recognition by thyroid hormone and retinoic acid receptors: 3,4,5 rule modified.

Molecular and Cellular Endocrinology
Theresa Q PhanMartin A Privalsky

Abstract

It has been proposed that retinoic acid receptors (RARs) and thyroid hormone receptors (TRs) both bind to AGGTCA "half-site" sequences, but distinguish their different target genes by recognizing different half-site spacings. We report here that artificial DNA binding sites based on these AGGTCA half-sites confer high affinity, but poor specificity, and that spacing alone does not account for the divergent DNA recognition properties of TRs and RARs. Instead, we have determined that the non-consensus half-sites that are present in naturally occurring RAR and TR target genes play a crucial role in defining receptor DNA recognition specificity, and work together with flanking sequences and half-site spacing to produce receptor-specific DNA binding in vitro. We also provide evidence that auxiliary proteins in cells generate an additional layer of receptor-specific target gene recognition, in part by destabilizing the binding of nuclear receptors to the "wrong" response elements.

Citations

Dec 1, 2012·Molecular & Cellular Proteomics : MCP·Qiongming LiuBei Zhen
Sep 5, 2012·The Journal of Clinical Investigation·Gregory A Brent
Feb 10, 2012·The Journal of Clinical Endocrinology and Metabolism·Alfonso Massimiliano FerraraSamuel Refetoff
May 24, 2013·PloS One·Lourdes Garrido-SanchezFrancisco J Tinahones
Sep 5, 2013·Thyroid : Official Journal of the American Thyroid Association·Antonio C BiancoUNKNOWN American Thyroid Association Task Force on Approaches and Strategies to Investigate Thyroid Hormone Economy and Action
Jul 16, 2011·Journal of Thyroid Research·Meghan D Rosen, Martin L Privalsky
Aug 20, 2014·Nature Reviews. Endocrinology·Tânia M Ortiga-CarvalhoFredric E Wondisford
Dec 15, 2010·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Eric Fulkerson, Patricia Ann Estes
Aug 14, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Grace S LeeMichael D Collins
Aug 20, 2014·Biochimica Et Biophysica Acta·José Xavier-NetoHozana Andrade Castillo
Jan 7, 2015·Nature Reviews. Molecular Cell Biology·Thomas J Cunningham, Gregg Duester
Mar 10, 2021·Biology of Sex Differences·Shounak Baksi, Ajay Pradhan
Oct 31, 2017·Endocrinology and Metabolism Clinics of North America·Moray J Campbell, Donald L Trump
Feb 1, 2019·Molecular and Cellular Endocrinology·Helder Veras Ribeiro FilhoAna Carolina Migliorini Figueira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.